関連する実験動画
Updated: May 8, 2026

12:19
Analysis of Cell Cycle Position in Mammalian Cells
Published on: January 21, 2012
Cdc25の活性化に必要なサイクリンBのドメインであるPボックスの機能分析
1Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, Massachusetts 02115.
Cell
|October 8, 1993
まとめ
サイクリンBのPボックスドメインは,cdc25フォスファタゼの活性化に不可欠です. この発見は,細胞サイクル中のサイクリンB-cdc2複合体の活性化のための2段階のメカニズムを支持する.
科学分野:
- 細胞生物学 細胞生物学
- 分子生物学は分子生物学である.
- バイオケミストリー バイオケミストリー
背景:
- サイクリンB-cdc2複合体は,細胞サイクル進行を調節する.
- 阻害性リン酸化は,G2の後期までcdc2不活性化を維持する.
- フォスファタゼcdc25は,M相へのエントリーのためにサイクリンB-cdc2複合体を活性化します.
研究 の 目的:
- cdc25の活性化におけるサイクリンBPボックスの役割を調査する.
- cdc25依存サイクリンB-cdc2活性化のメカニズムを解明する.
主な方法:
- 変異したサイクリンBタンパク質のcdc2.2とのインビトロ結合測定法.
- 細胞サイクル停止フェノタイプの分析.
- 野生型および変異性サイクリンB.の存在下におけるcdc25フォスファタゼ活性評価
主要な成果:
- 変異したPボックスを持つ変異サイクリンBタンパク質は,cdc2を結合するが,cdc25を活性化することができない.
- 変異性サイクリンBタンパク質を発現する細胞は,G2で細胞サイクル停止を示す.
- cdc25フォスファテーゼの活動は,サイクリンB Pボックスに依存し,インターフェーズとMフェーズ形態の異なる調節がある.
結論:
- サイクリンBのPボックスドメインは,cdc25の活性化に不可欠です.
- サイクリンB-cdc2複合体のcdc25依存活性化のための2段階メカニズムがサポートされています.
- この研究は,細胞サイクル制御における重要な規制のステップを明確にします.
関連する概念動画
Positive Regulator Molecules
To consistently produce healthy cells, the cell cycle—the process that generates daughter cells—must be precisely regulated.
Positive Regulator Molecules
Mitotic cell division results in daughter cells that exactly resemble the parent cell. However, errors in the DNA replication or distribution of genetic material may lead to genetic mutations that may be passed down to every new cell formed from the resulting abnormal cell. Propagation of such mutant cells is restricted through checkpoint mechanisms present at different stages of the cell cycle. These checkpoints involve regulator molecules that either promote or demote cell cycle events.
Inhibition of Cdk Activity
The orderly progression of the cell cycle depends on the activation of Cdk protein by binding to its cyclin partner. However, the cell cycle must be restricted when undergoing abnormal changes. Most cancers correlate to the deregulated cell cycle, and since Cdks are a central component of the cell cycle, Cdk inhibitors are extensively studied to develop anticancer agents. For instance, cyclin D associates with several Cdks, such as Cdk 4/6, to form an active complex. The cyclin D-Cdk4/6 complex...
Anaphase Promoting Complex
The stepwise destruction of specific proteins is necessary for the progression and completion of the cell cycle. Such proteins are ubiquitinated by ubiquitin ligases and then subsequently destroyed by the proteasome. The SCF (Skp1/Cullin/F-box) and the anaphase-promoting complex (APC) are two important ubiquitin ligases involved in cell cycle progression. While SCF is active throughout the cell cycle, APC gets activated during metaphase to anaphase transition. Cdc20 or Cdh1 binds to APC and...
Inhibition of CDK Activity
The orderly progression of the cell cycle depends on the activation of Cdk protein by binding to its cyclin partner. However, the cell cycle must be restricted when undergoing abnormal changes. Most cancers correlate to the deregulated cell cycle, and since Cdks are a central component of the cell cycle, Cdk inhibitors are extensively studied to develop anticancer agents. For instance, cyclin D associates with several Cdks, such as Cdk 4/6, to form an active complex. The cyclin D-Cdk4/6 complex...
Anaphase Promoting Complex
The stepwise destruction of specific proteins is necessary for the progression and completion of the cell cycle. Such proteins are ubiquitinated by ubiquitin ligases and then subsequently destroyed by the proteasome. The SCF (Skp1/Cullin/F-box) and the anaphase-promoting complex (APC) are two important ubiquitin ligases involved in cell cycle progression. While SCF is active throughout the cell cycle, APC gets activated during metaphase to anaphase transition. Cdc20 or Cdh1 binds to APC and...

