Tra-2の3'非翻訳領域によるトランスレーション制御は,C. elegansの性的アイデンティティを制御する
E B Goodwin1, P G Okkema, T C Evans
1Laboratory of Molecular Biology, University of Wisconsin-Madison 53706.
Cell
|October 22, 1993
まとめ
tra-2遺伝子の直接的な繰り返す要素 (DREs) は,その翻訳を制御し,C. elegansがハーマフロディットとして発達することを保証します. DREsを破壊すると,精子のないヘルマフロディットが生じ,トランスレーションの調節が強調される.
科学分野:
- 発達生物学 発達生物学とは
- 遺伝学 遺伝学とは
- 分子生物学は分子生物学である.
背景:
- ネマトードC.elegansは,ヘルマフロディット性繁殖系を示し,最初に精子を産み,次に卵子を産生する.
- C. elegansの性別決定は,その生命周期にとって極めて重要であり,tra-2のような遺伝子が重要な役割を果たしています.
研究 の 目的:
- tra-2遺伝子の3'未翻訳領域に位置する直接繰り返す要素 (DREs) の機能を調査する.
- DREsが翻訳レベルでのtra-2遺伝子発現を調節するメカニズムを解明する.
主な方法:
- 野生型および変異株におけるtra-2メッセンジャーRNA (mRNA) とのポリソーム関連性の分析.
- DREsを含むレポーターRNAを用いたインビトロ翻訳アッセイ.
- DRE障害後の tra-2 mRNA レベルの評価.
主要な成果:
- トラ-2 (tra-2(gf)) の機能獲得変異により,精子のないヘルマフロディットが生み出され,雌に変形します.
- DREsは,tra-2遺伝子翻訳を抑制し,mRNAの安定性またはレベルに影響を与えないことが判明しました.
- RNA結合因子はDREsと特異的に関連しており,直接的な規制相互作用を示唆しています.
結論:
- DREsによるtra-2の翻訳抑制は,C. elegansがハーマフロディット/雄性種として適切に発達するために不可欠です.
- RNA結合因子のDREsとの相互作用は,tra-2.の翻訳制御を媒介する.
- この研究は,性決定と発達に不可欠な翻訳的調節の新しいメカニズムを明らかにしています.
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