プロトオンコプロテインRelとTATA結合タンパク質との関連は,NF-kappa Bによる転写活性化を媒介する
L D Kerr1, L J Ransone, P Wamsley
1Molecular Biology and Virology Laboratory, Salk Institute, San Diego, California 92138.
Nature
|September 30, 1993
まとめ
c-Relタンパク質は,TATA結合タンパク質 (TBP) と相互作用して,遺伝子転写を促進します. 誤ったTBP結合がプロセスを阻害するので,この相互作用は極めて重要であり,エンハンサーと基礎転写因子の間の直接的なリンクを示唆する.
科学分野:
- 分子生物学は分子生物学である.
- 遺伝学 遺伝学とは
- バイオケミストリー バイオケミストリー
背景:
- c-Relタンパク質は,遺伝子発現の調節に関与する重要な転写因子です.
- TATA結合タンパク質 (TBP) は TFIID複合体の重要な構成要素であり,基礎転写の開始に不可欠である.
研究 の 目的:
- c-RelとTBPの相互作用を調査する.
- カッパB媒介遺伝子トランザクティベーションにおけるこの相互作用の機能的意義を決定する.
主な方法:
- タンパク質の相互作用を研究するためのインビトロおよびインビボ結合アッセイ.
- トランザクティベーションを評価するために,ドロソフィラ・シュナイダー細胞における共表現実験.
- TBPのDNA結合変異体の分析により,c-Rel機能への影響を評価する.
主要な成果:
- c-Relタンパク質は,in vitroとin vivoの両方でTBPと関連しています.
- TBPとc-Relの共発は,kappa Bサイトからのトランザクティベーションを高めます.
- DNA結合に欠陥のあるTBP変異体は,c-Relとの協力に失敗するだけでなく,c-Rel媒介のトランザクティベーションを抑制します.
結論:
- c-Rel と TBP の間には直接的な機能的なリンクが存在します.
- カッパB増強剤結合タンパク質とTBPのような基礎転写因子との相互作用は,増強された転写に極めて重要です.
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