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Generation and Culturing of Primary Human Keratinocytes from Adult Skin
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エピダーマルのランゲルハンズ細胞が,E-カデリンによって媒介されたケラチノサイトに付着する
A Tang1, M Amagai, L G Granger
1Dermatology Branch, National Cancer Institute, Bethesda, Maryland 20892.
Nature
|January 7, 1993
まとめ
皮膚のランゲルハンズ細胞 (LC) は,ケラチノサイト (KC) に粘着するためにE-カデリンを使用します. 培養LCにおけるE-カデリンの減少は,皮膚からリンパ節への移動を説明する可能性がある.
科学分野:
- 免疫学 免疫学とは
- 皮膚科 皮膚科について
- 細胞生物学 細胞生物学
背景:
- ランゲルハンズ細胞 (LC) は,表皮の重要な免疫細胞であり,補助細胞として機能します.
- 皮質の再定植は,LCの限られた自己再生能力のために,骨髄由来LCの祖先に依存しています.
- LCは,抗原被曝時に皮膚からリンパ節に移行するが,そのメカニズムは不明である.
研究 の 目的:
- LC密輸におけるランゲルハンズ細胞-ケラチノサイト (LC-KC) 相互作用の役割を調査する.
- LCが表皮細胞に接着する際の分子メカニズムを特徴づける.
主な方法:
- 新鮮および培養のマウリンLCにおけるカデリン発現の分析.
- LC-KC粘着のインビトロ評価.
- E-カデリン濃度の比較と,新鮮なLCと培養LCの粘着相性
主要な成果:
- 新鮮なネズミのLCはカデリンを発現し,E-カデリン経由でケラチノサイト (KC) に結合する.
- 培養されたLCは,移住した細胞に似ている可能性があるが,E-カデリンの発現が低下している.
- 培養されたLCは,新鮮なLCと比較して,KCに対する粘着親和性が低下しています.
結論:
- LCにおけるE-カデリンの発現は,皮膜内でのそれらの保持に決定的な役割を果たしているようです.
- カデリンは,白血球-表皮の相互作用において,これまで認識されていない重要な役割を果たす可能性があります.
- LC-KC粘着メカニズムの理解は,免疫細胞の密輸と保持に関する洞察を提供します.
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