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Updated: Aug 12, 2026

PCR Mutagenesis, Cloning, Expression, Fast Protein Purification Protocols and Crystallization of the Wild Type and Mutant Forms of Tryptophan Synthase
09:31

PCR Mutagenesis, Cloning, Expression, Fast Protein Purification Protocols and Crystallization of the Wild Type and Mutant Forms of Tryptophan Synthase

Published on: September 26, 2020

アクティブ・センター・トルション・アングル・ストレンは,ヒスティジンを含むフォスフォキャリアタンパク質の1.6A解像度構造で明らかになった

Z Jia1, M Vandonselaar, J W Quail

  • 1Department of Chemistry, University of Saskatchewan, Saskatoon, Canada.

Nature
|January 7, 1993
PubMed
まとめ

ヒスティジンを含有するフォスフォキャリアタンパク質 (HPr) 構造は,その活性部位でユニークなトルション・アングル・ストレインを明らかにします. この菌株は,活性化エネルギー障壁を低下させ,細菌の炭水化物輸送をフォスフォトランスフェラーゼ系を通じて促進する可能性がある.

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