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Mad: マックスのヘテロディメアパートナーで,マイクスの転写活動に敵対する
D E Ayer1, L Kretzner, R N Eisenman
1Division of Basic Sciences, Fred Hutchinson Cancer Research Center, Seattle, Washington 98104.
Cell
|January 29, 1993
まとめ
研究者らは,マックスと連携する新しいタンパク質"マッド"を発見した. このマッド・マックス・コンプレックスは,マイク・マックスと同じく,DNAを結合するが,反対の機能を持ち,マックスを明らかにする.
科学分野:
- 分子生物学は分子生物学である.
- 遺伝学 遺伝学とは
- タンパク質の相互作用
背景:
- Mycタンパク質は,マックスと異体化する転写因子である.
- 他の調節タンパク質の機能を媒介するマックスの役割は完全に理解されていません.
研究 の 目的:
- マックスと相互作用する新しいタンパク質を特定する.
- マックス相互作用タンパク質の機能とDNA結合特性を特徴付ける.
主な方法:
- ラジオラベル付マックスタンパク質を用いたラムダ gt11 エクスプレッションライブラリのスクリーニング.
- タンパク質とタンパク質の相互作用を評価するためのインビトロ結合測定法.
- In vivoトランスアクティベーションアッセイでは,転写活動の評価を行う.
主要な成果:
- マックスに結合する新しいbHLH-Zipタンパク質,Madの特定.
- Myc-Maxに類似したDNA結合特性を持つMad-Maxヘテロダイマーの形成.
- マイクマックス複合体とマッドマックス複合体は,マックスホモダイマーよりも優れ,CKIIリン酸化の影響を受けません.
- マッド・マックスとマイク・マックス複合体は,転写において対極的な機能を発揮する.
結論:
- マックスは,マイクとマッドを巻き込んだ転写因子のネットワークの中心的な仲介者です.
- マッドはマックスの新しい機能的パートナーであり,マイクスの逆の転写的役割を担っている.
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