Mxi1は,特にMaxと相互作用して,Myc-Maxの認識部位と結合するタンパク質です
A S Zervos1, J Gyuris, R Brent
1Department of Genetics, Harvard Medical School, Boston, Massachusetts 02114.
Cell
|January 29, 1993
まとめ
研究者らは,マックスに結合する新しいタンパク質,マックスインタラクター1 (Mxi1) を発見した. Mxi1-Max複合体は,Mycタンパク質の機能を阻害し,細胞の分化と癌に潜在的に影響を与える可能性があります.
科学分野:
- 分子生物学は分子生物学である.
- タンパク質対タンパク質の相互作用
- 遺伝子規制 遺伝子規制
背景:
- Myc族のタンパク質は,細胞の成長と分化に重要な役割を果たします.
- マックスは,Mycタンパク質の二分化パートナーとして知られており,DNAに結合する複合体を形成します.
- Maxと相互作用するタンパク質を理解することは,Myc媒介の遺伝子調節を解読する鍵です.
研究 の 目的:
- マックスと特異的に相互作用する新しいヒトタンパク質を特定する.
- このような相互作用するタンパク質の機能的性質を特徴付ける.
- Mycファミリータンパク質の機能を調節するこれらの相互作用の役割を明らかにする.
主な方法:
- 相互作用トラップ技術を活用して,マックスと相互作用するタンパク質を分離しました.
- 構造的モチーフ,特にbHLH-Zipドメインを特定したタンパク質を分析しました.
- 相互作用と結合部位の特異性を確認するために,共免疫プレシピテーションとDNA結合分析を行った.
- 酵母における転写活性を評価し,ヒトの細胞系におけるmRNA発現レベルを測定した.
主要な成果:
- マックスインタラクター1 (Mxi1) と名付けられた新しいヒトタンパク質を分離し,特定しました.
- Mxi1はbHLH-Zipモチーフを持ち,Mycファミリータンパク質に似ている.
- Mxi1は特にMaxと異体分解し,Myc-MaxのコンセンサスDNA認識部位と結合する複合体を形成する.
- Mxi1-Maxヘテロダイマーは転写を刺激せず,Mxi1 mRNAの発現はミエロイド白血病細胞の分化において上昇する.
結論:
- Mxi1は,Max.の特定のインタラクターとして作用する.
- Mxi1-Maxヘテロジメは,Maxを隔離し,DNA結合部位に競争することによって,Myc機能を阻害することができます.
- Mxi1はMyc主導のプロセスに阻害的な役割を果たし,細胞の分化に潜在的に影響を与える可能性があります.
関連する概念動画
03:17Inheritance of Chromatin Structures
6.0K
Epigenetics is the study of inherited changes in a cell's phenotype without changing the DNA sequences. It provides a form of memory for the differential gene expression pattern to maintain cell lineage, position-effect variegation, dosage compensation, and maintenance of chromatin structures such as telomeres and centromeres. For example, the structure and location of the centromere on chromosomes are epigenetically inherited. Its functionality is not dictated or ensured by the underlying...
6.0K
02:04Co-activators and Co-repressors
7.0K
Gene transcription is regulated by the synergistic action of several proteins that form a complex at a gene regulatory site. This is observed in eukaryotes, where the regulation of gene expression is a complex process. Regulatory proteins in eukaryotes can broadly be classified into two types – regulators that bind directly to specific DNA sequences and co-regulators that associate with regulatory proteins but cannot directly bind to the DNA. These co-regulators are further divided into...
7.0K
02:23Master Transcription Regulators
6.1K
Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...
6.1K
02:23Abnormal Proliferation
4.0K
Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
4.0K
01:07MAPK Signaling Cascades
7.3K
Mitogen-activated protein kinase, or MAPK pathway, activates three sequential kinases to regulate cellular responses such as proliferation, differentiation, survival, and apoptosis. The canonical MAPK pathway starts with a mitogen or growth factor binding to an RTK. The activated RTKs stimulate Ras, which recruits Raf or MAP3 Kinase (MAPKKK), the first kinase of the MAPK signaling cascade. Raf further phosphorylates and activates MEK or MAP2 Kinases (MAPKK), which in turn phosphorylates MAP...
7.3K
01:06Induced Pluripotent Stem Cells
4.8K
Stem cells are undifferentiated cells that divide and produce different cell types. Ordinarily, cells that have differentiated into a specific cell type are terminally differentiated; however, scientists have found a way to reprogram these mature cells so that they dedifferentiate and return to an unspecialized, proliferative state. These cells are pluripotent like embryonic stem cells—able to produce all cell types—and are called induced pluripotent stem cells (iPSCs).
Somatic...
Somatic...
4.8K