関連する実験動画
Updated: Oct 6, 2026

07:44
High-throughput Purification of Affinity-tagged Recombinant Proteins
Published on: August 26, 2012
ドロソフィラ転写因子IIDの最大のサブユニットは,TBPを含む複合体とコアクティベーターを含む複合体の組み立てを指揮する
R O Weinzierl1, B D Dynlacht, R Tjian
1Howard Hughes Medical Institute, Department of Molecular and Cell Biology, University of California, Berkeley 94720.
Nature
|April 8, 1993
まとめ
研究者は,ドロソフィラ TAFII250をクローンし,部分的なTFIID複合体を組み立てました. TBP,TAFII110およびTAFII250を含むこの複合体は,Sp1の活性化をサポートし,重要な分子相互作用を明らかにします.
科学分野:
- 分子生物学は分子生物学である.
- 遺伝学 遺伝学とは
- バイオケミストリー バイオケミストリー
背景:
- TFIID複合体は,遺伝子転写に不可欠であり,レギュレータによる活性化を媒介する.
- TATA結合タンパク質 (TBP) とTATA関連因子 (TAF) を含む.
研究 の 目的:
- ドロソフィラTAFII250をクローンし,TFIID複合体内のその役割を特徴づける.
- TBP,TAFII110およびTAFII250.0との相互作用を調査する.
主な方法:
- ドロソフィラのクローン TAFII250.0.
- 再結合部分TFIID複合体 (TBP, TAFII110, TAFII250 C端末ドメイン) の組み立てについて.
- Sp1活性化のための機能的測定法.
主要な成果:
- ドロソフィラ TAFII250.0.のクローニングが成功しました.
- Sp1の活性化をサポートする機能的なトリプル複合体の組み立て.
- TAFII250,TBP,TAFII110.の間の特定の相互作用を特定する
結論:
- クローン化されたDrosophila TAFII250は,TFIID複合体の機能的な構成要素である.
- 部分複合体は,TFIIDの組立と機能の洞察を提供します.
- TFIID複合体内の特定の相互作用は,転写の調節に極めて重要です.
関連する概念動画
Transcription Factors
Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...
Transcription Factors
Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...
Co-activators and Co-repressors
Gene transcription is regulated by the synergistic action of several proteins that form a complex at a gene regulatory site. This is observed in eukaryotes, where the regulation of gene expression is a complex process. Regulatory proteins in eukaryotes can broadly be classified into two types – regulators that bind directly to specific DNA sequences and co-regulators that associate with regulatory proteins but cannot directly bind to the DNA. These co-regulators are further divided into...
Transcription Initiation
Initiation is the first step of transcription in eukaryotes. Prokaryotic RNA Polymerase (RNAP) can bind to the template DNA and start transcribing. On the other hand, transcription in eukaryotes requires additional proteins, called transcription factors, to first bind to the promoter region in the DNA template. This binding helps recruit the specific RNAP that can assemble on the DNA and start transcription.
The promoters and enhancers and their accessory proteins allow tight regulation of...
The promoters and enhancers and their accessory proteins allow tight regulation of...
General Transcription Factors
Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...
Co-activators and Co-repressors
Gene transcription is regulated by the synergistic action of several proteins that form a complex at a gene regulatory site. This is observed in eukaryotes, where the regulation of gene expression is a complex process. Regulatory proteins in eukaryotes can broadly be classified into two types – regulators that bind directly to specific DNA sequences and co-regulators that associate with regulatory proteins but cannot directly bind to the DNA. These co-regulators are further divided into...

