Epidermal成長因子はp21rasを,受容体,Grb2アダプタータンパク質,Sos核酸交換因子の複合体の形成を通じて調節する
1Signal Transduction Laboratory, Imperial Cancer Research Fund, Lincoln's Inn Fields, London, England.
Cell
|May 7, 1993
まとめ
皮膜成長因子 (EGF) 信号は,Ras信号の重要な調節体である7less (Sos) タンパク質のSonを活性化します. この活性化には,Grb2アダプタータンパク質を通じてSosをEGF受容体に誘導する.
科学分野:
- 分子生物学は分子生物学である.
- 細胞シグナル伝達 細胞信号伝達
- バイオケミストリー バイオケミストリー
背景:
- Son of sevenless (Sos) は,p21ras.に対するグアニン核酸交換因子である.
- 皮膚表皮成長因子 (EGF) 受容体のシグナル伝達は,細胞の成長と分化に不可欠です.
- Grb2は,信号伝導に関与するSH2およびSH3ドメインを含むアダプタータンパク質です.
研究 の 目的:
- EGFがSOSの核酸交換活動を刺激するメカニズムを明らかにする.
- Grb2とEGF受容体のSOS活性化における役割を調査する.
主な方法:
- コイムノプレシピテーションは,タンパク質とタンパク質の相互作用を検出するための測定法です.
- タンパク質複合体の組成を評価するための in vitro 再構成実験.
- 浸透性細胞システムアッセイは,核酸交換活動を測定する.
主要な成果:
- SOSはEGF受容体とEGF刺激細胞で共免疫的に発生する.
- Grb2は,活性化されたEGF受容体とSosとの相互作用に不可欠です.
- EGF受容体のチロシン1068のフォスフォペプチドは,複合体の形成と下流信号伝達を阻害する.
結論:
- EGFによって誘発されるp21ras核酸交換の活性化には,Sosをプラズマ膜複合体に勧誘する.
- この複合体にはEGF受容体とGrb2アダプタータンパク質が含まれています.
- この相互作用は,EGF受容体の特定のリン酸化イベントによって媒介されます.
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