ヒトのETS1-DNA複合体の溶液構造は,結合の新しいモードと真のサイドチェーンのインターカレーションを明らかにします
M H Werner1, M Clore, C L Fisher
1Laboratory of Chemical Physics, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland 20892-0520, USA.
Cell
|December 1, 1995
まとめ
人間のETS1オンコプロテインである.
科学分野:
- 分子生物学は分子生物学である.
- 構造生物学 構造生物学とは
- バイオケミストリー バイオケミストリー
背景:
- 人間のETS1オンコタンパク質は,細胞のプロセスに役割を果たします.
- そのDNA結合メカニズムを理解することは,その機能を理解するために極めて重要です.
研究 の 目的:
- ヒトETS1 (hETS1) のDNAと複合したDNA結合ドメイン (DBD) の溶液構造を解明する.
- hETS1.で採用されているヘリックス・ターン・ヘリックス (HTH) -DNA相互作用のユニークなモードを特徴づける.
主な方法:
- 核磁共振 (NMR) スペクトロスコピーは,hETS1 DBD-DNA複合体の3D構造を決定するために使用されました.
- タンパク質とDNAの接触を理解するために,構造データの詳細な分析.
主要な成果:
- hETS1のDNA結合ドメイン (DBD) は,新しいヘリックス・ターン・ヘリックス (HTH) メカニズムを通じてDNAと相互作用する.
- 認識には,HTH認識ヘリックスによる主要な溝結合と,トリプトファン側鎖によるマイナー溝間隔が含まれる.
- この相互作用はDNAに重大な歪みを誘導し,その小さな溝の幅を変更します.
結論:
- DNA結合タンパク質のETSファミリーは,HTHタンパク質の独特のクラスを表しています.
- hETS1のユニークなDNA結合モードは,オンコタンパク質としての機能に意味を持っています.
関連する概念動画
01:16The DNA Helix
129.4K
Overview
129.4K
01:49Conserved Binding Sites
4.1K
Many proteins’ biological role depends on their interactions with their ligands, small molecules that bind to specific locations on the protein known as ligand-binding sites. Ligand-binding sites are often conserved among homologous proteins as these sites are critical for protein function.
Binding sites are often located in large pockets, and if their location on a protein’s surface is unknown, it can be predicted using various approaches. The energetic method computationally...
Binding sites are often located in large pockets, and if their location on a protein’s surface is unknown, it can be predicted using various approaches. The energetic method computationally...
4.1K
02:05DNA as a Genetic Template
22.2K
Two structural features of the DNA molecule provide a basis for the mechanisms of heredity: the four nucleotide bases and its double-stranded nature. The Watson-Crick model of double-helical DNA structure, proposed in 1952, drew heavily upon the X-ray crystallography work of researchers Rosalind Franklin and Maurice Wilkins. Watson, Crick, and Wilkins jointly received the Nobel Prize in Physiology or Medicine for their work in 1962. Franklin was, controversially, excluded from the prize for...
22.2K
02:54Nucleosome Remodeling
8.7K
Nucleosomes are the basic units of chromatin compaction. Each nucleosome consists of the DNA bound tightly around a histone core, which makes the DNA inaccessible to DNA binding proteins such as DNA polymerase and RNA polymerase. Hence, the fundamental problem is to ensure access to DNA when appropriate, despite the compact and protective chromatin structure.
Nucleosome remodeling complex
Eukaryotic cells have specialized enzymes called ATP-dependent nucleosome remodeling enzymes. These enzymes...
Nucleosome remodeling complex
Eukaryotic cells have specialized enzymes called ATP-dependent nucleosome remodeling enzymes. These enzymes...
8.7K
01:03Single-Strand DNA Binding Proteins
12.9K
For successful DNA replication, the unwinding of double-stranded DNA must be accompanied by stabilization and protection of the separated single strands of the DNA. This crucial task is performed by single-strand DNA-binding (SSB) proteins. They bind to the DNA in a sequence-independent manner, which means that the nitrogenous bases of the DNA need not be present in a specific order for binding of SSB proteins to it. The binding of SSB proteins straightens single-stranded DNA (ssDNA) and makes...
12.9K
01:07The DNA Helix
19.9K
Deoxyribonucleic acid, or DNA, is the genetic material responsible for passing traits from generation to generation in all organisms and most viruses. DNA is composed of two strands of nucleotides that wind around each other to form a spring-like structure called a double helix. However, the double helix is not perfectly symmetrical. Instead, there are regularly occurring grooves in the structure. The major groove occurs where the sugar-phosphate backbones are relatively far apart. This space...
19.9K