信号伝導のエチレン誘導性成分で,決して再現されない方法で暗号化されています
J Q Wilkinson1, M B Lanahan, H C Yen
1Monsanto Company, Chesterfield, MO 63198, USA.
Science (New York, N.Y.)
|December 15, 1995
まとめ
Never-ripe (Nr) トマト変異種は,重要な植物ホルモンであるエチレンに無感である. NR遺伝子の突然変異である.
科学分野:
- 植物生物学 植物生物学
- 分子遺伝学 分子遺伝学
- ホルモンのシグナル伝達
背景:
- Never-ripe (Nr) ミュータントは,エチレン不敏感性のために果物の熟成が損なわれている.
- エチレンは,成熟を含む様々な発達過程を調節する重要な植物ホルモンです.
研究 の 目的:
- Never-ripe (Nr) トマトの変異体におけるエチレン無感性の遺伝的根拠を調査する.
- Never-ripe (NR) 遺伝子とそのタンパク質産物について説明する.
主な方法:
- 遺伝子分析により,Nr現象型の原因となる遺伝子を特定する.
- 分子クローニングとNR遺伝子の配列決定.
- 変異性トマト植物における変異性NR遺伝子の発現.
主要な成果:
- NR遺伝子は,エチレン受容体と同型のタンパク質をコードしているが,応答調節ドメインが欠けている.
- NRタンパク質のセンサー領域における単一のアミノ酸置換は,エチレン無感性を授与する.
- NR遺伝子発現レベルは,トマトの果実の熟成時に調節されます.
結論:
- NR遺伝子は,トマトのエチレンシグナル伝達経路の重要な構成要素です.
- 特定された突然変異は,エチレン受容体の機能とエチレン媒介による果物の発達に関する洞察を提供します.
- NR遺伝子発現を調節することは,熟成中のエチレン反応を制御する重要な要因です.
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