bHLHタンパク質のサブクラスで,心臓の形態生成に必要とされる

D Srivastava¹, P Cserjesi, E N Olson

¹Department of Biochemistry and Molecular Biology, University of Texas M. D. Anderson Cancer Center, Houston 77030, USA.

Science (New York, N.Y.)

|December 22, 1995

まとめ

筋肉特有の2つの転写因子,dHANDとeHANDは,脊椎動物の心臓発育に不可欠である. 共に,心臓管の形成を調節し,胚性心臓の形態変異における冗長な役割を示唆する.

科学分野:

発達生物学発達生物学とは
分子遺伝学分子遺伝学

背景:

骨格筋の発達は,基本的なヘリックス・ループ・ヘリックス (bHLH) 転写因子に依存しています.
特定のbHLH遺伝子の早期心臓発達の役割は完全に理解されていません.

研究の目的:

脊椎動物の胚形成におけるdHANDとeHAND遺伝子の機能を調査する.
心臓発育におけるdHANDとeHANDの関与を判断する.

主な方法:

dHANDとeHAND遺伝子の分離と特徴付けについて.
チキンとマウスの胚における表情分析.
ステージ8のチキの胚に対するアンチセンセスのオリゴヌクレオチド処理.

主要な成果:

dHANDとeHANDは,発達中の心臓のプライモルディアとチューブル型の心臓で表現されます.
dHANDまたはeHANDのためのアンチセンスオリゴヌクレオチドは,単独では開発に影響を与えませんでした.
結合されたdHANDとeHANDの反感覚オリゴヌクレオチドは,循環する心臓管の段階での胚の発達を止めました.

結論:

dHANDとeHANDは,脊椎動物の心臓の発達を調節する上で不可欠で潜在的に冗長な役割を果たしています.
これらの遺伝子は,心臓管の形成につながる形態遺伝的イベントに不可欠です.

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