MEF2とミオジェニックのbHLHタンパク質による筋肉遺伝子発現の協同活性化
J D Molkentin1, B L Black, J F Martin
1Department of Biochemistry and Molecular Biology University of Texas M. D. Anderson Cancer Center, Houston 77030, USA.
Cell
|December 29, 1995
まとめ
ミオサイト増強因子-2 (MEF2) とミオジェニック・ベーシック・ヘリックス・ループ・ヘリックス (bHLH) タンパク質は,ミオゲネシスを強化するために協力します. そのDNA結合ドメインの間の直接的な相互作用により,新たな間接的な遺伝子活性化メカニズムが生まれます.
科学分野:
- 分子生物学は分子生物学である.
- 発達生物学 発達生物学について
- 遺伝学 遺伝学とは
背景:
- ミオサイト増強因子-2 (MEF2) タンパク質はMADSドメインの転写因子である.
- MyoDやmyogeninなどのMyogenic basic-helix-loop-helix (bHLH) タンパク質は,筋肉発達の重要なレギュレーターである.
研究 の 目的:
- ミオゲネシスに対するMEF2とミオジェニックのbHLHタンパク質の協力効果を調査する.
- この協力の基礎となる分子機構を解明する.
主な方法:
- MEF2とミオジェニックのbHLHタンパク質の共発は,感染した線維芽細胞で発生する.
- ミオジェニック変換の範囲の分析.
- DNA結合ドメイン間のタンパク質-タンパク質相互作用の調査.
主要な成果:
- MEF2とミオジェニックのbHLHタンパク質は,フィブロブラストにおけるミオジェニック変換を相乗的に増加させる.
- 協力には,MEF2のDNA結合ドメインと肌性bHLH因子の直接の相互作用が必要です.
- 協同活性化のためにトランザクティベーションドメインまたはDNA結合活性を必要とするのは,タンパク質パートナーの1つだけです.
結論:
- MEF2とミオジェニックのbHLHタンパク質は,ミオゲネシスの促進において協力性を示す.
- 異なった転写因子クラス間のタンパク質-タンパク質相互作用を通じて,間接的な遺伝子活性化の新しいメカニズムが明らかにされています.
- この相互作用により,結合部位の交叉活性化が可能になり,転写出力を強化します.
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