RNAポリメラーゼIIホロ酵素には,クロマチンの改造に関与するSWI/SNFレギュレータが含まれています
C J Wilson1, D M Chao, A N Imbalzano
1Whitehead Institute for Biomedical Research Nine Cambridge Center, Massachusetts 02142, USA.
Cell
|January 26, 1996
まとめ
SWI/SNFタンパク質は,酵母RNAポリメラーゼIIホロ酵素に不可欠であり,グローバル遺伝子調節体として作用する. これらの成分はクロマチンを改造し,転写開始複合体のプロモーターへの結合を促進します.
科学分野:
- 分子生物学は分子生物学である.
- 遺伝学 遺伝学とは
- バイオケミストリー バイオケミストリー
背景:
- RNAポリメラーゼIIホロ酵素は,遺伝子転写に不可欠な複合体である.
- 一般的な転写因子とSRBタンパク質は,このホロ酵素の既知の成分である.
- SWIとSNFの遺伝子産物は,クロマチンの改造に関与する確立されたグローバルな遺伝子調節体である.
研究 の 目的:
- イーストRNAポリメラーゼIIホロ酵素におけるSWIおよびSNF遺伝子産物の役割を調査する.
- SWI/SNFタンパク質がホロ酵素の不可欠な成分であるかどうかを判断する.
- SWI/SNFタンパク質がホロ酵素の機能にどのように貢献するのかを理解する.
主な方法:
- イーストRNAポリメラーゼIIホロ酵素の生化学分析.
- ホロ酵素複合体内のタンパク質成分を識別する.
- SWI/SNFタンパク質の機能的貢献を評価するためのアッセイ.
主要な成果:
- SWIおよびSNF遺伝子製品は,酵母RNAポリメラーゼIIホロ酵素の不可欠な成分として特定されました.
- これらのSWI/SNFタンパク質は,RNAポリメラーゼII C末端の繰り返し領域に関連したSRB複合体 (媒介体) の一部である.
- SWI/SNFの成分は,ホロ酵素が核細胞DNAを破壊することを可能にします.
結論:
- SWI/SNFタンパク質は,酵母RNAポリメラーゼIIホロ酵素の不可欠な成分であり,その既知の機能をグローバル遺伝子調節を超えて拡張しています.
- SWI/SNFタンパク質の含有により,ホロ酵素はクロマチンの構造を改造する能力を得ます.
- このクロマチンの改造能力は,プロモーター領域での転写開始複合体の安定した組み立てを促進し,転写効率を高めます.
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