ホメオドメインタンパク質によるRNA認識と翻訳的調節
1Howard Hughes Medical Research Institute, Department of Genetics and Development, Columbia University College of Physicians and Surgeons, New York, New York 10032, USA.
Nature
|February 22, 1996
まとめ
ドロソフィラのビコイド (bcd) タンパク質は遺伝子を活性化するだけでなく,RNAを結合させ,尾関節 (cad) 翻訳を抑制します. この二重の機能は,胚の発達に不可欠な対極の形態原 gradients を確立します.
科学分野:
- 発達生物学 発達生物学とは
- 分子遺伝学 分子遺伝学
- ゲノミクスゲノミクスとは
背景:
- ビコイド (bcd) モルフォゲン・グラデーションは,ドロソフィラ胚の前部パターニングに不可欠である.
- bcdは転写因子として機能し,濃度値に基づいて標的遺伝子を活性化します.
研究 の 目的:
- 尾関節 (cad) 遺伝子の調節におけるビコイド (bcd) の役割を調査する.
- bcdの潜在的RNA結合能力とそのCAD翻訳への影響について調査する.
主な方法:
- ビコイド (bcd) タンパク質と尾 (cad) mRNAとの相互作用の分析.
- bcdによる翻訳的抑圧のメカニズムを調査する.
主要な成果:
- ビコイド (bcd) が尾部 (cad) のメッセンジャーRNAに直接結合することを示す証拠があります.
- bcdは翻訳抑制剤として作用し,CADタンパク質合成を阻害する.
- この相互作用は,cad 3' 未翻訳領域の特定の配列に bcd ホメオドメインの結合を伴う.
結論:
- ビコイド (bcd) は,転写活性化と転写抑制という二重の機能を持っています.
- cad翻訳のbcd媒介抑制は,対極の形態原グラデントを確立するのに寄与する.
- このメカニズムは,胚の前後パターンにおける新しい遺伝子調節層を提供する.
関連する概念動画
02:24Regulation of Expression Occurs at Multiple Steps
Gene expression can be regulated at almost every step from gene to protein. Transcription is the step that is most commonly regulated. This involves the binding of proteins to short regulatory sequences on the DNA. This association can either promote or inhibit the transcription of a gene associated with the respective sequence.
Transcription results in the generation of precursor (pre-mRNA) that consists of both exons and introns, which needs further processing before being translated to a...
Transcription results in the generation of precursor (pre-mRNA) that consists of both exons and introns, which needs further processing before being translated to a...
02:23Master Transcription Regulators
Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...
02:24Regulation of Expression Occurs at Multiple Steps
Gene expression can be regulated at almost every step from gene to protein. Transcription is the step that is most commonly regulated. This involves the binding of proteins to short regulatory sequences on the DNA. This association can either promote or inhibit the transcription of a gene associated with the respective sequence.
Transcription results in the generation of precursor (pre-mRNA) that consists of both exons and introns, which needs further processing before being translated to a...
Transcription results in the generation of precursor (pre-mRNA) that consists of both exons and introns, which needs further processing before being translated to a...
02:23Master Transcription Regulators
Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...
01:23Regulation of Expression at Multiple Steps
The gene expression in cells is regulated at different stages: (i) transcription, (ii) RNA processing, (iii) RNA localization, and (iv) translation. Transcriptional regulation is mediated by regulatory proteins such as transcription factors, activators, or repressors—these control gene expression by initiating or inhibiting the transcription of genes. Once a precursor or pre-mRNA is produced, it undergoes post-transcriptional modification, including 5' capping, splicing, and the addition of a...
01:29Translational Regulation
Translational regulation in prokaryotes ensures efficient protein synthesis by controlling ribosome access to mRNA. This regulation is mediated by secondary RNA structures, including translational riboswitches, RNA thermometers, and small RNAs (sRNAs), which respond to intracellular and environmental signals to modulate gene expression.Translational RiboswitchesRiboswitches in the leader region of mRNAs can regulate translation by altering the accessibility of the Shine-Dalgarno (SD) sequence,...