SRBとDNA修復タンパク質に関連したヒトRNAポリメラーゼII複合体

E Maldonado¹, R Shiekhattar, M Sheldon

¹Howard Hughes Medical Institute, Wood Johnson Medical School, University of Medicine and Dentistry of New Jersey, Piscataway 08854-5635, USA.

Nature

|May 2, 1996

まとめ

研究者は,基礎転写因子とSRBタンパク質を含むヒトRNAポリメラーゼII複合体を分離した. この複合体は遺伝子転写を強化し,DNA修復に関与しています.

科学分野:

分子生物学は分子生物学である.
バイオケミストリーバイオケミストリー
遺伝学遺伝学とは

背景:

RNAポリメラーゼIIは,遺伝子転写の中心に位置しています.
基礎転写因子とSRBタンパク質は,RNAポリメラーゼIIの活性を調節するために重要である.
人間のRNAポリメラーゼII複合体の正確な組成と機能はまだ解明されていない.

研究の目的:

人間のRNAポリメラーゼII複合体を分離し,特徴づけること.
転写活性化におけるこの複合体の役割を調査する.
転写とDNA修復メカニズムの間の潜在的なリンクを探求する.

主な方法:

RNAポリメラーゼIIを含むマルチタンパク質複合体の分離.
関連する基礎転写因子とSRBタンパク質の同類体の識別.
トランスクリプションの活性化とDNA修復活動を測定するアッセイ.

主要な成果:

ヒトRNAポリメラーゼII複合体を成功裏に分離しました.
複合体には,基礎転写因子とヒトSRBタンパク質の同種が含まれています.
孤立した複合体は,トランスクリプションの活性化が強化されたことを示した.
証拠によると,一部の成分がDNA修復に関与していることが示されています.

結論:

制御機能を持つ新しいヒトRNAポリメラーゼII複合体が特定されました.
この複合体は,転写の共同活性化に役割を果たします.
この発見は,転写とDNA修復経路の間の機能的なリンクを示唆しています.

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