SNARE媒介の逆行トラフィックは,ゴルギ複合体から,エンドプラズマ網膜への逆行トラフィックである
1MRC Laboratory of Molecular Biology, Cambridge, United Kingdom.
Cell
|April 19, 1996
まとめ
研究者らは,ゴルギからエンドプラズマ網膜への逆行輸送に不可欠な新しい酵母t-SNAREタンパク質を特定しました. このタンパク質は,特定の受容体と膜タンパク質のリサイクルに不可欠であり,細胞の交通に影響を与えます.
科学分野:
- 細胞生物学 細胞生物学
- 分子生物学は分子生物学である.
- タンパク質の密輸 タンパク質の密輸
背景:
- ユカリオットの分泌経路は,膀の輸送と融合に依存しています.
- v-SNAREsとt-SNAREsは,オーガネルのターゲティングを媒介する.
- 逆行輸送は,リサイクルに不可欠です.
研究 の 目的:
- 逆行輸送に関与する新しい酵母t-SNAREを特定し,特徴づけること.
- タンパク質と受容体のリサイクルにおけるこのt-SNAREの役割を明らかにする.
- 逆行経路における他のタンパク質の関与を理解する.
主な方法:
- イースト遺伝学とミュータント分析.
- 膜タンパク質とその回収信号の特徴.
- 膀輸送とオルガネルのターゲティングの分析.
主要な成果:
- エンドプラズマ網膜に新しい酵母 t-SNARE が特定されました.
- このt-SNAREの変異は,HDEL受容体と二基底回収信号タンパク質のリサイクルを阻害しました.
- 前進の交通は間接的に遮断され,後退の経路の役割を示唆しました.
結論:
- 新型t-SNAREは,ゴルギからERへの逆行輸送に不可欠です.
- Sec21p (gamma-COP) とSec20pも,主に逆行移動に関与しています.
- この研究は,酵母分泌経路の重要な構成要素を明らかにしています.
さらに関連する動画
関連する概念動画
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01:09Golgi Apparatus
Properly folded and assembled proteins are selectively packaged into vesicles that exit the ER. Motor proteins transport these vesicles to the Golgi apparatus for adding modifications that make these proteins functional at their destination.
The Golgi apparatus is a eukaryotic organelle that has a distinctive ribbon-like appearance. It is a primary sorting and dispatch station for cargo arriving from the ER. Newly arriving vesicles enter the cis face of the Golgi, closest to the ER, and are...
The Golgi apparatus is a eukaryotic organelle that has a distinctive ribbon-like appearance. It is a primary sorting and dispatch station for cargo arriving from the ER. Newly arriving vesicles enter the cis face of the Golgi, closest to the ER, and are...
01:32Export of Misfolded Proteins out of the ER
After folding, the ER assesses the quality of secretory and membrane proteins. The correctly folded proteins are cleared by the calnexin cycle for transport to their final destination, while misfolded proteins are held back in the ER lumen. The ER chaperones attempt to unfold and refold the misfolded proteins but sometimes fail to achieve the correct native conformation. Such terminally misfolded proteins are then exported to the cytosol by ER-associated degradation or ERAD pathway for...
01:26Transport Across the Golgi
While it is unclear how molecules move between adjacent Golgi cisternae, it is apparent that the molecules move from cis- cisterna, the entry face, to the trans- cisterna, the exit face. Experiments initially suggested vesicles that bud from one cisterna and fuse with the next cisterna to transport proteins between the cisternae. This vesicular transport model describes the Golgi apparatus as a relatively static structure with a unique enzyme composition in each cisterna. Molecules are...
01:09Golgi Apparatus
Properly folded and assembled proteins are selectively packaged into vesicles that exit the ER. Motor proteins transport these vesicles to the Golgi apparatus for adding modifications that make these proteins functional at their destination.
The Golgi apparatus is a eukaryotic organelle that has a distinctive ribbon-like appearance. It is a primary sorting and dispatch station for cargo arriving from the ER. Newly arriving vesicles enter the cis face of the Golgi, closest to the ER, and are...
The Golgi apparatus is a eukaryotic organelle that has a distinctive ribbon-like appearance. It is a primary sorting and dispatch station for cargo arriving from the ER. Newly arriving vesicles enter the cis face of the Golgi, closest to the ER, and are...