複製の連結は,Cdk阻害剤Sic1によって開始される
B L Schneider1, Q H Yang, A B Futcher
1Cold Spring Harbor Laboratory, Cold Spring Harbor, NY 11724, USA.
Science (New York, N.Y.)
|April 26, 1996
まとめ
この研究は,Sic1の無活性化が酵母細胞分裂におけるG1サイクリン (Clns) の重要な役割であることを明らかにしています. ClnsによるSic1のリン酸化と分解は,Start移行時のDNA複製のコミットメントを調節する.
科学分野:
- 細胞生物学 細胞生物学
- 分子生物学は分子生物学である.
- イースト遺伝学 イースト遺伝学
背景:
- 3つのG1サイクリン (Clns) は,Saccharomyces cerevisiaeのスタートイベントを調節し,細胞を分裂させます.
- Sic1はC1b-Cdc28キナーゼの既知の阻害体であり,細胞サイクル進行に不可欠である.
研究 の 目的:
- 酵母細胞分裂におけるG1サイクリン (Clns) の重要な機能を明らかにする.
- スタート移行におけるSic1のリン酸化と分解の役割を調査する.
主な方法:
- 特定のサイクリンとSic1.を欠くSaccharomyces cerevisiae変異体の分析
- Cln活動に関連してSic1のリン酸化と分解を調査する.
主要な成果:
- スタート時のSic1のリン酸化は,Clnの活性に依存する.
- Sic1の分解には,Clnsとウビキチン結合酵素Cdc34.4を必要とする.
- Sic1の非活性化がClnsの唯一の非冗長な基本機能であり,sic1の削除はcln1 cln2 cln3のトリプルミュータントを救う.
- sic1変異体は,結合されていないDNA複製と芽生えを示しています.
結論:
- Sic1はCln-Cdc28複合体の基板である可能性が高い.
- ClnsによるSic1のリン酸化およびその後のタンパク質分解は,Start移行におけるDNA複製へのコミットメントを調節するために重要である.
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