神経成長因子は,記憶Bリンパ球の自己決定性生存因子である
M Torcia1, L Bracci-Laudiero, M Lucibello
1Department of Experimental Medicine, University of Rome "Tor Vergata", Italy.
Cell
|May 3, 1996
まとめ
神経成長因子 (NGF) は,記憶Bリンパ球の自己決定性生存因子として作用する. NGFを阻害すると,メモリB細胞が死亡し,体内での抗体生成が低下する.
科学分野:
- 免疫学 免疫学とは
- 細胞生物学 細胞生物学
- 神経科学は神経科学である.
背景:
- 神経成長因子 (NGF) は,神経細胞の発達と生存に不可欠です.
- リンパ球機能,特にB細胞生存におけるNGFの役割は完全に理解されていません.
研究 の 目的:
- 人間のリンパ球におけるNGFの生成と機能を調査する.
- NGFがBリンパ球,特に記憶B細胞の生存因子として作用するかどうかを判断する.
主な方法:
- NGFの生産を評価するためにヒトTおよびBリンパ球およびマクロファージの培養.
- NGFに対する中和抗体を利用して,リンパ球の生存と機能に及ぼす影響を研究する.
- リンパ球におけるBcl-2タンパク質レベルとアポプトシス細胞死亡を測定する.
- 特定の抗原で免疫を施したマウスの体内試験で,抗体位数とB細胞集団に対する抗NGF抗体の影響を評価した.
主要な成果:
- B細胞はNGFを構成的に生成し,その受容体 (p140trk-Aとp75NGFR) を発現する.
- 内生NGFの中和は,メモリBリンパ球 (IgG+またはIgA+) でアポプトシス死を引き起こすが,ネイブBリンパ球 (IgM+/IgD+) ではそうではない.
- 免疫化されたマウスの体内でのNGF抗体の投与は,特定のIgG抗体の位数とIgG+またはIgA+B細胞の数を低下させた.
結論:
- NGFは,メモリBリンパ球に特化したオトクリン生存因子です.
- NGFは,抗体を産生するB細胞の生存を維持し,体内免疫を調節する上で重要な役割を果たします.
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