CBPインテグレーター複合体は,核受容体による転写活性化とAP-1抑制を媒介する
1Howard Hughes Medical Institute, School of Medicine, University of California, San Diego, La Jolla, 92093-0648, USA.
Cell
|May 3, 1996
まとめ
核受容体はCREB結合タンパク質 (CBP) を活性化するために必要であり,CBP/p300の競争を通じてAP-1を抑制する. CBPは核の複数のシグナル伝達経路を統合し,核受容体,CREB,AP-1の共通因子として作用する.
科学分野:
- 分子生物学は分子生物学である.
- 遺伝子規制 遺伝子規制
- バイオケミストリー バイオケミストリー
背景:
- 核受容体は遺伝子発現の重要な調節体である.
- 標的遺伝子を活性化し,AP-1の活性を抑制する.
- これらの機能の基礎となる正確なメカニズムは複雑です.
研究 の 目的:
- 核受容体媒介遺伝子調節におけるCREB結合タンパク質 (CBP) の役割を調査する.
- 核受容体がAP-1活動を抑制するメカニズムを解明する.
- 核内の複数のシグナル伝達経路を統合する共通の要因を特定する.
主な方法:
- コイムノプレシピテーションアッセイは,タンパク質の相互作用を研究するためのものです.
- 細胞における核受容体とAP-1活性に関する分析.
- 核受容体共活性剤のクローン化と特徴化.
主要な成果:
- 予期せぬことに,核受容体の活性化には,CREB結合タンパク質 (CBP) が必要です.
- AP-1の活性抑制は,限られたCBP/p300の競争によるものと思われる.
- CBPは核受容体とp160共同活性化体 (SRC-1変種) と直接相互作用する.
結論:
- CBPは,核受容体,CREB,AP-1の機能に不可欠な共通因子である.
- CBP/p300は,複数の核信号伝導経路のインテグレーターとして機能します.
- SRC-1の変種のような明確なコアクティベーターは,核受容体の機能のためにCBPと連携する.
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