酵母菌のCDC16およびCDC27遺伝子は,DNA複製を細胞周期ごとに1回に制限する
1Division of Basic Sciences, Fred Hutchinson Cancer Research Center, Seattle, Washington 98104, USA.
Cell
|April 5, 1996
まとめ
酵母菌における新しい遺伝子であるCDC16とCDC27は,DNA複製を制限する. ミュータントは1つの細胞サイクル内で8CまでDNAを過剰複製し,細胞サイクル制御におけるその役割を強調する.
科学分野:
- 分子生物学は分子生物学である.
- 細胞生物学 細胞生物学
- 遺伝学 遺伝学とは
背景:
- DNA複製は,細胞周期ごとに1回しか発生しないように厳格に規制されなければならない.
- 細胞サイクル制御システムは,DNAの過剰複製を防止することによって,ゲノムの安定性を確保します.
- 複製制御に関与する遺伝子の理解は,細胞サイクル研究にとって極めて重要です.
研究 の 目的:
- DNA複製を調節するSaccharomyces cerevisiaeの遺伝子を特定する.
- DNAの過剰複製が起こるメカニズムを調査する.
- DNA複製に影響を与える新しい条件アルレルの特徴を特定する.
主な方法:
- S. cerevisiae. のDNA過剰複製変異体に対する遺伝子スクリーニング
- DNA含有量の分析 (例えば,8C DNAの蓄積).
- ミトーシスとSTARTのモニタリングを含む細胞サイクル分析.
- チェックポイントのアクティベーションを調査する (例えば,MEC1チェックポイント).
- サイクリン依存キナーゼ活性 (例えば,Clb2-Cdc28) を評価する.
主要な成果:
- CDC16とCDC27は,DNA複製を細胞周期1回に1回に制限するために不可欠な遺伝子として特定されました.
- スクリーンでは,DNAの過剰複製 (8Cまで) を引き起こす新しい条件アレルが特定されました.
- 過剰複製は,すべての染色体を含む1つの細胞サイクル内で発生し,MEC1チェックポイントを誘発する可能性があります.
- Clb2-Cdc28の活性が高まった状態は,過剰複製する細胞で持続した.
- これらの発見は,異変性ミトーシスによる過剰複製を引き起こす変異体からCDC16/CDC27の役割を区別する.
結論:
- CDC16とCDC27は,S. cerevisiaeのDNA過剰複製を防止する上で重要な役割を果たしています.
- これらの遺伝子は,S相イニシアターの標的タンパク質分解を調節することによって機能する可能性があります.
- この研究は,細胞サイクル制御とDNA複製の信頼性の分子機構に関する新しい洞察を提供します.
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In eukaryotes, the initiation of replication occurs at many sites on the chromosomes, called the origins of replication.
02:36DNA Damage Can Stall the Cell Cycle
In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...
02:38S-Cdk Initiates DNA Replication
The cell cycle is a series of events leading to DNA duplication followed by the division of cell content to form two daughter cells. The cell cycle progresses in four stages—the cell increases in size (gap 1 or G1-phase), duplicates its DNA (synthesis or S-phase), prepares to divide (gap 2 or G2-phase), and divides (mitosis or M-phase).
Two states at the origin of replication
In eukaryotes, the initiation of replication occurs at many sites on the chromosomes, called the origins of replication.
Two states at the origin of replication
In eukaryotes, the initiation of replication occurs at many sites on the chromosomes, called the origins of replication.