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関連する概念動画

The Ras Gene02:38

The Ras Gene

5.7K
The Ras-gene-encoded proteins are regulators of signaling pathways controlling cell proliferation, differentiation, or cell survival. The Ras-gene family in humans constitutes three primary members—the HRas, NRas, and KRas. These genes code for four functionally distinct yet closely related proteins—the HRas, NRas, KRas4A, and KRas4B. The involvement of mutant Ras genes in human cancer was first discovered in 1982 and is among the most common causes of human tumorigenesis.
Ras is a...
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Small GTPases - Ras and Rho01:24

Small GTPases - Ras and Rho

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Ras and Rho are small monomeric GTPases that act downstream of receptor tyrosine kinase (RTK) and regulate various cellular processes. These GTPases switch between active and inactive states by binding to guanine nucleotides.
Three regulatory proteins control their activity:
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MAPK Signaling Cascades01:07

MAPK Signaling Cascades

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Mitogen-activated protein kinase, or MAPK pathway, activates three sequential kinases to regulate cellular responses such as proliferation, differentiation, survival, and apoptosis. The canonical MAPK pathway starts with a mitogen or growth factor binding to an RTK. The activated RTKs stimulate Ras, which recruits Raf or MAP3 Kinase (MAPKKK), the first kinase of the MAPK signaling cascade. Raf further phosphorylates and activates MEK or MAP2 Kinases (MAPKK), which in turn phosphorylates MAP...
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Integrins01:10

Integrins

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Animal and protozoan cells do not have cell walls to help maintain shape and provide structural stability. Instead, these eukaryotic cells secrete a sticky mass of carbohydrates and proteins into the spaces between adjacent cells. This network of proteins and molecules is called an extracellular matrix or ECM.
Some ECM proteins assemble into a basement membrane to which the remaining components adhere. Proteoglycans typically form the bulk of the ECM while fibrous proteins, like collagen,...
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Activation of Integrins01:15

Activation of Integrins

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Integrins bind ligands and transmit information from outside the cell to inside or vice-versa through an "outside-in signaling" or "inside-out signaling."
In "outside-in signaling," external factors in the extracellular space bind to exposed ligand binding sites on integrins. This causes the inactive protein to undergo a conformational change to become active. Integrins are often clustered on the cell membrane. Repetitive and regularly spaced ligand binding...
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Intracellular Signaling Affects Focal Adhesions01:17

Intracellular Signaling Affects Focal Adhesions

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Integrins act both as extracellular input receivers and as intracellular processing activators. As their name suggests, integrins are entirely integrated into the membrane structure. Their hydrophobic membrane-spanning regions interact with the phospholipid bilayer's hydrophobic region. These membrane receptors provide extracellular attachment sites for effectors like hormones and growth factors. They activate intracellular response cascades when their effectors are bound and active.
Some...
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関連する実験動画

Updated: May 5, 2026

RhoC GTPase Activation Assay
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RhoC GTPase Activation Assay

Published on: August 23, 2010

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R-rasによるインテグリン活性化.

Z Zhang1, K Vuori, H Wang

  • 1La Jolla Cancer Research Center, The Burnham Institute, California 92037, USA.

Cell
|April 5, 1996
PubMed
まとめ
この要約は機械生成です。

活性化されたR-rasシグナリングは,インテグリン結合親和性とフィブロネクチンマトリックスアセンブリの強化により,サスペンション細胞を粘着細胞に変換します. この研究は,R-rasを明らかにしています.

さらに関連する動画

Spatio-Temporal Manipulation of Small GTPase Activity at Subcellular Level and on Timescale of Seconds in Living Cells
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Spatio-Temporal Manipulation of Small GTPase Activity at Subcellular Level and on Timescale of Seconds in Living Cells

Published on: March 9, 2012

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Characterize Disease-related Mutants of RAF Family Kinases by Using a Set of Practical and Feasible Methods
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Characterize Disease-related Mutants of RAF Family Kinases by Using a Set of Practical and Feasible Methods

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関連する実験動画

Last Updated: May 5, 2026

RhoC GTPase Activation Assay
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RhoC GTPase Activation Assay

Published on: August 23, 2010

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Spatio-Temporal Manipulation of Small GTPase Activity at Subcellular Level and on Timescale of Seconds in Living Cells
10:27

Spatio-Temporal Manipulation of Small GTPase Activity at Subcellular Level and on Timescale of Seconds in Living Cells

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Characterize Disease-related Mutants of RAF Family Kinases by Using a Set of Practical and Feasible Methods
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科学分野:

  • 細胞生物学 細胞生物学
  • 分子生物学は分子生物学である.
  • バイオケミストリー バイオケミストリー

背景:

  • インテグリンは,細胞粘着を媒介する重要な細胞表面受容体です.
  • R-rasは,細胞信号伝達経路に関与する小さなGTPaseである.
  • インテグリン活性の調節は,細胞のプロセスに不可欠です.

研究 の 目的:

  • インテグリン媒介細胞結合の調節におけるR-rasの役割を調査する.
  • R-rasがインテグリン機能に影響を与えるメカニズムを解明する.

主な方法:

  • 構成的に活性な,支配的な負のR-ras変異体を持つ細胞系を感染させる.
  • インテグリンリガンドに対する細胞粘着の評価.
  • インテグリン-リガンド結合親和性の測定.
  • フィブロネクチンマトリックスアセンブリの定量化.

主要な成果:

  • 構成的に活性なR-ras発現は,サスペンション細胞における高い細胞粘着を誘発した.
  • 活性化されたR-rasは,インテグリン結合親和性とフィブロネクチンマトリックス形成を高めました.
  • 支配的な負のR-rasは,内生的なR-ras媒介細胞の粘着性を低下させた.

結論:

  • R-rasは,インテグリンリンリンガンド結合活性を調節する上で重要な役割を果たします.
  • アクティベーションされたR-rasは,インテグリン機能を向上させることで,細胞粘着を促進します.
  • 固有のR-rasは,固有のインテグリン粘着性を調節し,新しい調節メカニズムを示唆します.