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転写因子AP-2は,頭蓋骨の閉塞と頭蓋骨顔面の発達に不可欠です
H Schorle1, P Meier, M Buchert
1Whitehead Institute for Biomedical Research, Cambridge, Massachusetts 02142, USA.
Nature
|May 16, 1996
まとめ
転写因子AP-2はマウスの胚の発達に不可欠である. AP-2遺伝子の変異は,重度の頭蓋骨と神経の欠陥を引き起こし,マウスの胎児間死亡を引き起こしました.
科学分野:
- 発達生物学 発達生物学とは
- 遺伝学 遺伝学とは
- 分子生物学は分子生物学である.
背景:
- 転写因子AP-2は,マウスのニューラルチューブ閉塞時にエクトダームとニューラルクレスト細胞で発現します.
- 頭蓋骨の神経頂部細胞は,頭蓋骨の発達,頭蓋骨の形成,頭蓋骨のギャングリアの発達に不可欠です.
研究 の 目的:
- 標的型ミュータゲネシスによるマウス胚形成におけるAP-2遺伝子の役割を調査する.
- AP-2遺伝子障害が胚の発達に及ぼす影響を理解する.
主な方法:
- マウスのAP-2遺伝子の標的型変異.
- AP-2ノックアウト (AP-2-/-) マウスのフェノタイプ分析.
主要な成果:
- AP-2ノックアウトマウスは,頭蓋骨-腹腔分裂による生後死亡率を示した.
- 深刻な頭蓋骨,頭蓋骨,感覚器官,頭蓋骨の状腺の異常が観察されました.
- 頭蓋の閉塞が9日から9.5日の間に起こっており,特定の脳領域のアポトーシスの増加と第1枝状門メセンキムに関連しています.
- ツイストとPax-3の表現は影響を受けませんでした.
結論:
- AP-2遺伝子は,マウスの正常な胚形成,特に頭部顔面および神経の発達に不可欠である.
- AP-2は,頭蓋骨神経管の閉塞と胚の発達中のアポトーシスの予防に重要な役割を果たします.
- 観察された欠陥は,twistまたはPax-3の表現の変化によるものではない.
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