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分裂酵母における姉妹染色体分離に必要なCut2タンパク質分解
H Funabiki1, H Yamano, K Kumada
1Department of Biophysics, Faculty of Science, Kyoto University, Japan.
Nature
|May 30, 1996
まとめ
Cut2タンパク質のアナフェーズ固有のタンパク質分解は,分裂酵母における姉妹染色体分離に不可欠である. その分解には,アミノ端末領域と,アナフェーズ促進複合体 (APC) の構成要素であるCut9が必要です.
科学分野:
- 細胞生物学 細胞生物学
- 分子生物学は分子生物学である.
- 遺伝学 遺伝学とは
背景:
- ミトスサイクリンは,メタフェーズからアナフェーズへの移行時にタンパク質分解をします.
- シスタークロマチドの分離にはサイクリン分解が不可欠ではない.
- このプロセスには,他のタンパク質のプロテオリシスが必要である可能性が高い.
研究 の 目的:
- Schizosaccharomyces pombe Cut2タンパク質のアナフェーズ固有のタンパク質分解を調査する.
- 姉妹染色体分離におけるCut2タンパク質分解の役割を決定する.
主な方法:
- 細胞サイクル中のCut2の局所化と分解を研究した.
- Cut2のアミノ端末領域とCut9の分解の必要性を調査した.
- 非分解性Cut2が細胞サイクル進行と姉妹染色体分離に及ぼす影響を調べました.
- 規制されたCut2タンパク質分解の必要性を実証するために,タンパク質移植技術を使用しました.
主要な成果:
- Cut2タンパク質は,アナフェーズ中に特異的に分解される.
- Cut2の分解には,そのアミノ末端領域とアナフェーズ促進複合体 (APC) のCut9.9成分が必要です.
- 非分解性Cut2の発現は,細胞サイクル進行に影響を与えることなく,姉妹染色体分離を阻害する.
- サイクリンBのN端をカットされたCut2に挿入すると,分離欠陥が解消される.
結論:
- Cut2の制御されたタンパク質分解は,分裂酵母における姉妹染色体分離に不可欠である.
- Cut2はAPCの重要な基板であり,細胞分裂の重要なステップを媒介する.
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