trpは,アゴニストで活性化されたキャパシタティブCa2+のエントリーに不可欠な新種の哺乳類の遺伝子ファミリーです
1Department of Anesthesiology, Department of Biological Chemistry, School of Medicine, Molecular Biology Institute, University of California, Los Angeles 90095-1778, USA.
Cell
|May 31, 1996
まとめ
容量性カルシウムエントリー (CCE) は,細胞反応に不可欠です. 哺乳類のtrp同種であるHtrp1とHtrp3は,カルシウム流入に不可欠なCCEチャネルの重要な構成要素として特定されました.
科学分野:
- 細胞生物学 細胞生物学
- 分子生理学 分子生理学
- イオンチャネル機能
背景:
- 容量性カルシウムエントリー (CCE) は,細胞シグナル伝達に不可欠であり,細胞内カルシウム貯蔵物を補充します.
- CCEの基礎となる分子機構は複雑で,完全に理解されていないため,複数のチャネルタイプが関与する可能性があります.
- ドロソフィラの研究により,CCEにおけるtrp遺伝子産物の役割が示唆された.
研究 の 目的:
- 容量性カルシウムエントリー (CCE) の分子基礎を調査する.
- CCEに関与する哺乳類の遺伝子を特定する.
- CCEチャネルのコンポーネントとしてtrpのホモログが機能するかどうかを判断する.
主な方法:
- マウスのゲノムをスクリーニングして,TRPに関連する遺伝子を検出した.
- CCEを抑制するために,L細胞の反感覚発現を利用した.
- CCEを調節するために,COS細胞でヒトのtrpホモログ (Htrp1,Htrp3) の全長cDNA発現を用いた.
主要な成果:
- マウスのゲノムで6つのtrp関連遺伝子を特定しました.
- これらの遺伝子の反意味断片の発現時に観察されたCCEの抑制.
- Htrp1およびHtrp3cDNAの発現は,COS細胞におけるCCEを著しく増加させた.
結論:
- 哺乳類のtrp同種,特にHtrp1とHtrp3は,CCEの機能的成分として特定されています.
- これらの発見は,trpホモログがCCEチャネルのサブユニットとして作用することを示唆しています.
- この研究は,細胞シグナル伝達に関与するカルシウム浸透性チャネルの分子構造の洞察を提供します.
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