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関連する概念動画

Plasmodesmata02:32

Plasmodesmata

30.0K
The organs in a multicellular organism’s body are made up of tissues formed by cells. To work together cohesively, cells must communicate. One way that cells communicate is through direct contact with other cells. The points of contact that connect adjacent cells are called intercellular junctions.
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Short-distance Transport of Resources02:12

Short-distance Transport of Resources

14.9K
Short-distance transport refers to transport that occurs over a distance of just 2-3 cells, crossing the plasma membrane in the process. Small uncharged molecules, such as oxygen, carbon dioxide, and water, can diffuse across the plasma membrane on their own. In contrast, ions and larger molecules require the assistance of transport proteins due to their charge or size. Transport across membranes also occurs within individual cells, playing a variety of essential roles for the plant as a whole.
14.9K
The Apoplast and Symplast01:46

The Apoplast and Symplast

51.4K
Plant growth depends on its ability to take up water and dissolved minerals from the soil. The root system of every plant is equipped with the necessary tissues to facilitate the entry of water and solutes. The plant tissues involved in the transport of water and minerals have two major compartments - the apoplast and the symplast. The apoplast includes everything outside the plasma membrane of living cells and consists of cell walls, extracellular spaces, xylem, phloem, and tracheids. The...
51.4K
Animal and Plant Cell Structure01:30

Animal and Plant Cell Structure

31.5K
Animal and plant cells not only differ in their structure, function, and mode of nutrition but also in how they reproduce, specialize, and organize into complex structures.
Cell Division
Though both plant and animal cells divide by mitosis (for non-gametic cells) and meiosis (for gametic cells), they differ in the specifics of this process. Unlike animal cells, plant cells lack centrosomes — an organelle responsible for organizing the spindle fibers and segregating the chromosomes during...
31.5K
Plant Cell Wall01:07

Plant Cell Wall

7.7K
Plant cells have a cell wall, a rigid outer covering that protects the cell and provides shape and support. During cell division, a mixture of enzymes, proteins, and glucose molecules is transported via vesicles to the center of the cell. These vesicles continuously fuse and build a cell plate between the dividing cells. As the cell plate matures, new polysaccharides are added to it to form the cell walls of the daughter cells. The predominant polysaccharide in the cell wall is cellulose, made...
7.7K
Plasmodesmata01:20

Plasmodesmata

3.8K
In a multicellular organism, cells must communicate to work together in a coordinated manner. One way that cells communicate is through direct contact with other cells. The points of contact that connect adjacent cells are called intercellular junctions.
Intercellular junctions are a feature of fungal, plant, and animal cells. However, different types of junctions are found in different kinds of cells. Intercellular junctions found in animal cells include tight junctions, gap junctions, and...
3.8K

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関連する実験動画

Updated: May 5, 2026

Preparation of Chloroplast Sub-compartments from Arabidopsis for the Analysis of Protein Localization by Immunoblotting or Proteomics
10:28

Preparation of Chloroplast Sub-compartments from Arabidopsis for the Analysis of Protein Localization by Immunoblotting or Proteomics

Published on: October 19, 2018

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植物細胞には,機能的に異なる2つの真空小区分が含まれています.

N Paris1, C M Stanley, R L Jones

  • 1Biochemistry Department, University of Missouri Columiba 65211, USA.

Cell
|May 17, 1996
PubMed
まとめ

植物細胞には,タンパク質の貯蔵とプロテアゼの活性のために異なる真空小区画が含まれています. アルファ-TIPとTIP-Ma27で示されたこれらのコンパートメントは,真空穴が成熟するにつれて結合し,貯蔵されたタンパク質の分解を可能にします.

科学分野:

  • 植物細胞生物学 植物細胞生物学
  • バキュオラ機能は,
  • プロテインの密輸,人身売買

背景:

  • 植物真空は,タンパク質の貯蔵や,プロテアース活性のための酸性pHの維持など,さまざまな機能を果たしています.
  • 以前は,これらの機能が単一の真空腔区間内で発生すると考えられていた.

研究 の 目的:

  • 植物細胞内の真空機能の分割を調査する.
  • 異なるトノプラスト固有タンパク質が異なる真空腔区画をマークしているかどうかを判断する.

主な方法:

  • トノプラスト固有タンパク質 (アルファ-TIPとTIP-Ma27) に対する抗体を用いた免疫マーキング.
  • 特定のタンパク質 (大麦のレクチンとアレウライン) の真空区画内の局所化を追跡する.

主要な成果:

  • アルファ-TIPとTIP-Ma27に対する抗体は,バキュール膜の異なるコンパートメントにラベルを付けています.
  • 大麦のレクチンは,アルファ-TIPコンパートメントのみで,アレウラインはTIP-Ma27コンパートメントで発見されました.
  • これらの異なるコンパートメントは,真空穴が成熟するにつれて融合します.

結論:

さらに関連する動画

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Author Spotlight: Microscopic Analysis of Protein Localization at Plasmodesmata in Plants
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Author Spotlight: Microscopic Analysis of Protein Localization at Plasmodesmata in Plants

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関連する実験動画

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Preparation of Chloroplast Sub-compartments from Arabidopsis for the Analysis of Protein Localization by Immunoblotting or Proteomics
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Visualizing Yeast Organelles with Fluorescent Protein Markers
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Visualizing Yeast Organelles with Fluorescent Protein Markers

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Author Spotlight: Microscopic Analysis of Protein Localization at Plasmodesmata in Plants
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Author Spotlight: Microscopic Analysis of Protein Localization at Plasmodesmata in Plants

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  • 植物真空は,特定のトノプラストタンパク質によって特徴づけられた,機能的に異なる区画で構成されています.
  • バクオールの発達中にこれらのコンパートメントの融合は,貯蔵されたタンパク質の分解を容易にする可能性があります.
  • この発見は,単一の,均一な真空孔の長期間の仮定に異議を唱える.