Cdc25細胞サイクルフォスファタゼは,c-myccの標的として使用されます
K Galaktionov1, X Chen, D Beach
1Howard Hughes Medical Institute, Cold Spring Harbor Laboratory, New York 11724, USA.
Nature
|August 8, 1996
まとめ
プロトオンコゲンであるc-mycとMaxは,細胞の成長と死を調節する転写因子を形成する. この因子は,Myc誘発のアポトーシスに不可欠なcdc25A遺伝子を標的にし,cdc25Aをc-mycにおける重要なプレーヤーとして強調しています.
科学分野:
- 分子生物学は分子生物学である.
- 腫瘍学 腫瘍学
- 細胞生物学 細胞生物学
背景:
- プロトオンコゲンc-mycは,マックスと共に転写因子を形成する.
- このMyc/Max複合体は,細胞増殖とアポトーシスに関与する遺伝子を調節する.
- また,原発がん遺伝子であるcdc25Aは,特に成長因子がない場合,アポトーシスにおいて役割を果たします.
研究 の 目的:
- c-mycとcdc25A遺伝子の関係を調査する.
- cdc25AがMyc/Max.の直接的な転写標的であるかどうかを判断する.
- Myc誘発のアポトーシスにおけるcdc25Aの役割を明らかにする.
主な方法:
- cdc25A遺伝子プロモーターに結合するMyc/Maxヘテロダイマーの分析.
- Myc/Max.によるcdc25A転写活性化の評価
- Myc誘発のアポトーシスに対するcdc25Aの要求の評価.
主要な成果:
- Myc/Maxヘテロダイマーは,cdc25A遺伝子内の特定の元素と結合する.
- Myc/Max結合は,cdc25A転写の活性化につながる.
- Mycによって誘発されるアポトーシスは,cdc25Aの存在に依存しています.
結論:
- cdc25Aは,c-myc.の生理学的に重要な転写標的である.
- Myc/Max複合体は,cdc25A発現を直接制御する.
- cdc25Aは,Myc誘発によるアポトーシスを媒介するために不可欠です.
関連する概念動画
02:34Inhibition of Cdk Activity
The orderly progression of the cell cycle depends on the activation of Cdk protein by binding to its cyclin partner. However, the cell cycle must be restricted when undergoing abnormal changes. Most cancers correlate to the deregulated cell cycle, and since Cdks are a central component of the cell cycle, Cdk inhibitors are extensively studied to develop anticancer agents. For instance, cyclin D associates with several Cdks, such as Cdk 4/6, to form an active complex. The cyclin D-Cdk4/6 complex...
02:15M-Cdk Drives Transition Into Mitosis
Checkpoints throughout the cell cycle serve as safeguards and gatekeepers, allowing the cell cycle to progress in favorable conditions and slow or halt it in problematic ones. This regulation is known as the cell cycle control system.
Cyclin-dependent kinases, or Cdks, work in concert with cyclins to control cell cycle transitions. M-Cdk, a complex of Cdk1 bound to M cyclin, is a well-known example of this coordinated control that drives the transition from the G2 to the M phase.
M cyclin...
Cyclin-dependent kinases, or Cdks, work in concert with cyclins to control cell cycle transitions. M-Cdk, a complex of Cdk1 bound to M cyclin, is a well-known example of this coordinated control that drives the transition from the G2 to the M phase.
M cyclin...
02:38Mitogens and the Cell Cycle
Mitogens and their receptors play a crucial role in controlling the progression of the cell cycle. However, the loss of mitogenic control over cell division leads to tumor formation. Therefore, mitogens and mitogen receptors play an important role in cancer research. For instance, the epidermal growth factor (EGF) - a type of mitogen and its transmembrane receptor (EGFR), decides the fate of the cell's proliferation. When EGF binds to EGFR, a member of the ErbB family of tyrosine kinase...
00:50Anaphase Promoting Complex
The stepwise destruction of specific proteins is necessary for the progression and completion of the cell cycle. Such proteins are ubiquitinated by ubiquitin ligases and then subsequently destroyed by the proteasome. The SCF (Skp1/Cullin/F-box) and the anaphase-promoting complex (APC) are two important ubiquitin ligases involved in cell cycle progression. While SCF is active throughout the cell cycle, APC gets activated during metaphase to anaphase transition. Cdc20 or Cdh1 binds to APC and...
02:34Inhibition of CDK Activity
The orderly progression of the cell cycle depends on the activation of Cdk protein by binding to its cyclin partner. However, the cell cycle must be restricted when undergoing abnormal changes. Most cancers correlate to the deregulated cell cycle, and since Cdks are a central component of the cell cycle, Cdk inhibitors are extensively studied to develop anticancer agents. For instance, cyclin D associates with several Cdks, such as Cdk 4/6, to form an active complex. The cyclin D-Cdk4/6 complex...
02:15M-Cdk Drives Transition Into Mitosis
Checkpoints throughout the cell cycle serve as safeguards and gatekeepers, allowing the cell cycle to progress in favorable conditions and slow or halt it in problematic ones. This regulation is known as the cell cycle control system.
Cyclin-dependent kinases, or Cdks, work in concert with cyclins to control cell cycle transitions. M-Cdk, a complex of Cdk1 bound to M cyclin, is a well-known example of this coordinated control that drives the transition from the G2 to the M phase.
M cyclin...
Cyclin-dependent kinases, or Cdks, work in concert with cyclins to control cell cycle transitions. M-Cdk, a complex of Cdk1 bound to M cyclin, is a well-known example of this coordinated control that drives the transition from the G2 to the M phase.
M cyclin...