関連する実験動画

Updated: Aug 15, 2026

DNA Polymerase Activity Assay Using Near-infrared Fluorescent Labeled DNA Visualized by Acrylamide Gel Electrophoresis
07:38

DNA Polymerase Activity Assay Using Near-infrared Fluorescent Labeled DNA Visualized by Acrylamide Gel Electrophoresis

Published on: October 6, 2017

ポリメラーゼ活性部位にあるDNAとTaqポリメラーゼの構造

S H Eom1, J Wang, T A Steitz

  • 1Department of Molecular Biophysics, Yale University, New Haven, Connecticut 06520-811, USA.

Nature
|July 18, 1996
PubMed
まとめ

研究者らは,活性部位のDNAに結合するTaqポリメラーゼの構造を決定した. これは,この酵素が,以前のKlenowの断片構造と異なる,ぼんやりしたDNAをどのように結合するかを明らかにします.

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PCR

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Translesion (TLS) polymerases rescue stalled DNA polymerases at sites of damaged bases by replacing the replicative polymerase and installing a nucleotide across the damaged site. Doing so, TLS allows additional time for the cell to repair the damage before resuming regular DNA replication.
TLS polymerases are found in all three domains of life - archaea, bacteria, and eukaryotes. Of the different classes of TLS polymerases, members of the Y family are fitted with specialized structures that...
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Nucleic Acid Structure

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