Jove
Visualize
お問い合わせ

関連する概念動画

Positive Regulator Molecules01:45

Positive Regulator Molecules

To consistently produce healthy cells, the cell cycle—the process that generates daughter cells—must be precisely regulated.
Positive Regulator Molecules02:39

Positive Regulator Molecules

Mitotic cell division results in daughter cells that exactly resemble the parent cell. However, errors in the DNA replication or distribution of genetic material may lead to genetic mutations that may be passed down to every new cell formed from the resulting abnormal cell. Propagation of such mutant cells is restricted through checkpoint mechanisms present at different stages of the cell cycle. These checkpoints involve regulator molecules that either promote or demote cell cycle events.
Inhibition of Cdk Activity02:34

Inhibition of Cdk Activity

The orderly progression of the cell cycle depends on the activation of Cdk protein by binding to its cyclin partner. However, the cell cycle must be restricted when undergoing abnormal changes. Most cancers correlate to the deregulated cell cycle, and since Cdks are a central component of the cell cycle, Cdk inhibitors are extensively studied to develop anticancer agents. For instance, cyclin D associates with several Cdks, such as Cdk 4/6, to form an active complex. The cyclin D-Cdk4/6 complex...
M-Cdk Drives Transition Into Mitosis02:15

M-Cdk Drives Transition Into Mitosis

Checkpoints throughout the cell cycle serve as safeguards and gatekeepers, allowing the cell cycle to progress in favorable conditions and slow or halt it in problematic ones. This regulation is known as the cell cycle control system.
Cyclin-dependent kinases, or Cdks, work in concert with cyclins to control cell cycle transitions. M-Cdk, a complex of Cdk1 bound to M cyclin, is a well-known example of this coordinated control that drives the transition from the G2 to the M phase.
M cyclin...
Inhibition of CDK Activity02:34

Inhibition of CDK Activity

The orderly progression of the cell cycle depends on the activation of Cdk protein by binding to its cyclin partner. However, the cell cycle must be restricted when undergoing abnormal changes. Most cancers correlate to the deregulated cell cycle, and since Cdks are a central component of the cell cycle, Cdk inhibitors are extensively studied to develop anticancer agents. For instance, cyclin D associates with several Cdks, such as Cdk 4/6, to form an active complex. The cyclin D-Cdk4/6 complex...
M-Cdk Drives Transition Into Mitosis02:15

M-Cdk Drives Transition Into Mitosis

Checkpoints throughout the cell cycle serve as safeguards and gatekeepers, allowing the cell cycle to progress in favorable conditions and slow or halt it in problematic ones. This regulation is known as the cell cycle control system.
Cyclin-dependent kinases, or Cdks, work in concert with cyclins to control cell cycle transitions. M-Cdk, a complex of Cdk1 bound to M cyclin, is a well-known example of this coordinated control that drives the transition from the G2 to the M phase.
M cyclin...

こちらも読む

関連記事

共著者、ジャーナル、引用グラフによってこの研究に関連する記事。

並び替え
Same author

Precision Measurement of Neutrino Oscillation Parameters with 10 Years of Data from the NOvA Experiment.

Physical review letters·2026
Same author

Defining Standard Data Reporting in Pelvic Exenterations for Non-Rectal Cancers: A Systematic Review of Current Data Reporting.

Cancers·2025
Same author

Mortality and Major Amputation in Patients With Diabetes-Related Foot Ulcers and Chronic Renal Disease.

ANZ journal of surgery·2025
Same author

Dual-Baseline Search for Active-to-Sterile Neutrino Oscillations in NOvA.

Physical review letters·2025
Same author

Search for CP-Violating Neutrino Nonstandard Interactions with the NOvA Experiment.

Physical review letters·2024
Same author

Abstracts of presentations to the Annual Meetings of the Canadian Society of Colon and Rectal Surgeons Canadian Association of General Surgeons Canadian Association of Thoracic Surgeons: Canadian Surgery Forum, London, Ont., Sept. 19 to 22, 2002.

Canadian journal of surgery. Journal canadien de chirurgie·2023
JoVE
x logofacebook logolinkedin logoyoutube logo
JoVEについて
概要リーダーシップブログJoVEヘルプセンター
著者向け
出版プロセス編集委員会範囲と方針査読よくある質問投稿
図書館員向け
推薦の声購読アクセスリソース図書館諮問委員会よくある質問
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experimentsアーカイブ
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教員リソースセンター教員サイト
利用規約
プライバシーポリシー
ポリシー

関連する実験動画

Updated: Jul 17, 2026

Assay for Adhesion and Agar Invasion in S. cerevisiae
04:36

Assay for Adhesion and Agar Invasion in S. cerevisiae

Published on: November 8, 2006

発芽酵母からのCdk活性化キナーゼ (CAK) です.

P Kaldis1, A Sutton, M J Solomon

  • 1Yale University School of Medicine, Department of Molecular Biophysics and Biochemistry, New Haven, Connecticut 06520-8024, USA.

Cell
|August 23, 1996
PubMed
まとめ

研究者は,酵母からCdk活性化キナーゼ (CAK) を浄化し,クローン化し,以前に研究されたCAKとは異なる機能をしていることを発見しました. この発見は,脊椎動物における生理学的CAKとしてのp40MO15-サイクリンH-MAT1の役割に挑戦しています.

科学分野:

  • 分子生物学は分子生物学である.
  • 細胞サイクル規制について
  • バイオケミストリー バイオケミストリー

背景:

  • サイクリン依存キナーゼ (CDK) の活性化は,細胞サイクル進行において極めて重要です.
  • この活性化には,サイクリンと特定のリン酸化,特にヒトのp34cdc2.2.におけるスレオニン残留161での結合が必要である.
  • Cdk活性化キナーゼ (CAK) は,この重要なリン酸化イベントに責任があります.

研究 の 目的:

  • Saccharomyces cerevisiae (酵母) からCAKを浄化しクローンするために.
  • 酵母 CAK (Cak1p) の性質を特徴付け,他の生物の CAK と比較する.
  • 遺伝的アプローチを用いて,細胞周期調節におけるCAKの生理学的役割を調査する.

主な方法:

  • S. cerevisiaeからCAKの浄化とクローニング.
  • イーストのCAKをE. coliで発現させ,その活性を評価する.
  • CAK1遺伝子における温度感受性突然変異の作成と分析.
  • 主要なミトックサイクリンをコードするCLB2遺伝子との遺伝的相互作用の研究.

主要な成果:

  • 酵母 CAK (Cak1p) は単体として活性であり,E. coliで発現すると完全な活性を示します.

さらに関連する動画

Identification of Cyclin-dependent Kinase 1 Specific Phosphorylation Sites by an In Vitro Kinase Assay
12:26

Identification of Cyclin-dependent Kinase 1 Specific Phosphorylation Sites by an In Vitro Kinase Assay

Published on: May 3, 2018

Manipulation and Analysis of Cell Cycle-Dependent Processes in Budding Yeast
08:13

Manipulation and Analysis of Cell Cycle-Dependent Processes in Budding Yeast

Published on: September 26, 2025

関連する実験動画

Last Updated: Jul 17, 2026

Assay for Adhesion and Agar Invasion in S. cerevisiae
04:36

Assay for Adhesion and Agar Invasion in S. cerevisiae

Published on: November 8, 2006

Identification of Cyclin-dependent Kinase 1 Specific Phosphorylation Sites by an In Vitro Kinase Assay
12:26

Identification of Cyclin-dependent Kinase 1 Specific Phosphorylation Sites by an In Vitro Kinase Assay

Published on: May 3, 2018

Manipulation and Analysis of Cell Cycle-Dependent Processes in Budding Yeast
08:13

Manipulation and Analysis of Cell Cycle-Dependent Processes in Budding Yeast

Published on: September 26, 2025

  • 他の種のCAKとは異なり,Cak1pは基礎転写因子TFIIHの一部ではありません.
  • CAK1の温度感受性突然変異は,G2細胞サイクル遅延,Cdc28pキナーゼ活性低下,CLB2との遺伝的相互作用につながる.

    • 結論:

    • 酵母 CAK (Cak1p) は,他の生物の CAK に比べて独特の生化学的および機能的特性を有しています.
    • この発見は,以前,脊椎動物のp40MO15-サイクリンH-MAT1複合体が,主な生理学的CAKではないことを示唆している.
    • この研究は,異なる種におけるCDK活性化の多様なメカニズムを理解するための新しい道を開きます.