Civ1 (CAK in vivo),新しいCdk活性化キナーゼである
J Y Thuret1, J G Valay, G Faye
1Service de Biochimie et Génétique Molécularie, Gif-sur-Yvette, France.
Cell
|August 23, 1996
まとめ
研究者らは,Civ1という新しいCdk活性化キナーゼ (CAK) を酵母菌で発見した. Civ1は細胞生存に不可欠であり,Cdc28をリン酸化して細胞分裂と遺伝子発現を調節する.
科学分野:
- 分子生物学は分子生物学である.
- 細胞生物学 細胞生物学
- バイオケミストリー バイオケミストリー
背景:
- サイクリン依存タンパク質キナーゼ (Cdks) は細胞分裂と遺伝子発現を調節する.
- Cdkの活性化には,通常,Cdk活性化キナーゼ (CAK) によってサイクリン結合とリン酸化が必要です.
研究 の 目的:
- Saccharomyces cerevisiae (S. cerevisiae) の新しいCAK活性を特定し,特徴づけること.
- この新しいCAKの生理学的関連性を in vivoで調査する.
主な方法:
- 潜在的なCAK候補者を特定するためのシーケンス類似性分析.
- バイオケミカルアッセイで,キナーゼ活性と基板特異性を確認する.
- 温度に敏感な変異体を用いた遺伝分析により,in vivoの機能を評価する.
主要な成果:
- Civ1 (CAK in vivo) の識別,Cdksと配列の類似性を持つ新しいCAKですが,モノマーとして活性です.
- Civ1はCdc28に直接結合し,リン酸化し,その後のサイクリン結合と活性化を可能にします.
- CIV1遺伝子は酵母生存に不可欠であり,civ1-4変異体はCdc28のリン酸化と活性に条件付きの欠陥を示しています.
結論:
- Civ1は,生体内での生理学的関連性に関する遺伝的証拠を持つ最初の特定されたCAKです.
- Civ1は,新しいキナーゼファミリーの創設メンバーを代表する可能性があります.
- Civ1は,S. cerevisiaeの細胞分裂と遺伝子発現を調節する上で重要な役割を果たしています.
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The cell cycle is a series of events leading to DNA duplication followed by the division of cell content to form two daughter cells. The cell cycle progresses in four stages—the cell increases in size (gap 1 or G1-phase), duplicates its DNA (synthesis or S-phase), prepares to divide (gap 2 or G2-phase), and divides (mitosis or M-phase).
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In eukaryotes, the initiation of replication occurs at many sites on the chromosomes, called the origins of replication.
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