XTcf-3転写因子は,Xenopus胚におけるβ-カタニン誘発の軸形成を媒介する

M Molenaar¹, M van de Wetering, M Oosterwegel

¹Department of Immunology University Hospital, Utrecht The Netherlands.

Cell

|August 9, 1996

まとめ

Xenopus Tcf-3 (XTcf-3) は,β-カタニンと提携し,それを核に転移して遺伝子転写を活性化します. このXTcf-3とβ-カテニンの相互作用は,胚軸の特異化にとって極めて重要です.

科学分野:

発達生物学発達生物学について
分子生物学は分子生物学である.
遺伝学遺伝学とは

背景:

XTcf-3は哺乳類のTcf-1とLef-1転写因子のXenopus同型である.
母体で発現するXTcf-3はβ-cateninと相互作用する.

研究の目的:

ベータ-カタニン媒介シグナル伝達と胚の発達におけるXTcf-3の役割を調査する.
転写活性化におけるXTcf-3のメカニズムを解明する.

主な方法:

XTcf-3 mRNAと,Xenopusの胚への支配的陰性変異体のマイクロインジェクション.
ベータ-カタニン相互作用アッセイ.
トランジエントレポーター遺伝子解析は,転写活性化のためのものです.
胚軸の仕様の分析. 胚軸の仕様の分析.

主要な成果:

XTcf-3は,そのN末端経由でβ-カタニンと結合する.
XTcf-3-β-catenin複合体は,β-cateninの核転移を誘導する.
この複合体は転写を活性化しますが,XTcf-3単独は不活性です.
N末端が欠けている支配的陰性XTcf-3変異体は,β-カタニン誘発の軸複製と内生軸形成を抑制する.

結論:

Xenopusにおけるβ-カテニンのシグナル伝達には,XTcf-3.で複雑な形成が含まれています.
XTcf-3-β-カタニン複合体の核転位は,特定の標的遺伝子を活性化させます.
XTcf-3は,ベータ-カタニン経路を通じた胚軸の特異化において重要な役割を果たします.

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