ベータカテニンのトランスクリプション因子LEF-1との機能的相互作用

J Behrens¹, J P von Kries, M Kühl

¹Max Delbrück Centre for Molecular Medicine, Berlin, Germany.

Nature

|August 15, 1996

まとめ

細胞質β-カテニンタンパク質は,転写因子であるリンパ性増強剤結合因子 (LEF-1) と相互作用する. この相互作用は遺伝子発現を調節し,細胞結合から細胞核への信号を伝達する.

科学分野:

分子生物学は分子生物学である.
発達生物学発達生物学とは
細胞シグナル伝達細胞信号伝達

背景:

ベータ-カテニンは細胞結合とWnt/Winglessシグナル伝達における二重の役割を持つ細胞質タンパク質です.
それはカデリン分子と細胞骨格を結びつけ,信号伝達経路に参加します.

研究の目的:

ベータ-カタニンと転写因子の相互作用を調査する.
細胞結合から遺伝子発現までの信号伝達の分子機構を解明する.

主な方法:

イースト2ハイブリッドスクリーニングで,相互作用するタンパク質を特定します.
哺乳類の細胞における共表現と免疫プレシピテーション.
DNAによる三元複合体の形成の分析.
Xenopus胚マイクロインジェクションアッセイ.

主要な成果:

リンパ性増強剤結合因子 (LEF-1) をβ-カタニン相互作用タンパク質として特定した.
LEF-1とβ-cateninの間の核複合体の形成が実証されました.
LEF-1とβ-カテニンはDNAと三元複合体を形成し,DNAの折り曲げを変化させます.
ベータ-カタニンによって強化されたXenopus胚のLEF-1-誘発の軸複製が観察されました.

結論:

ベータ-カタニンは,LEF-1のような転写因子との相互作用を通じて,遺伝子発現を直接調節する.
この相互作用は,細胞粘着成分またはWntタンパク質から核への信号伝送のための分子リンクを提供します.

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