Jove
Visualize
お問い合わせ
JoVE
x logofacebook logolinkedin logoyoutube logo
JoVEについて
概要リーダーシップブログJoVEヘルプセンター
著者向け
出版プロセス編集委員会範囲と方針査読よくある質問投稿
図書館員向け
推薦の声購読アクセスリソース図書館諮問委員会よくある質問
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experimentsアーカイブ
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教員リソースセンター教員サイト
利用規約
プライバシーポリシー
ポリシー

関連する概念動画

pre-mRNA Processing02:01

pre-mRNA Processing

In eukaryotic cells, transcripts made by RNA polymerase are modified and processed before exiting the nucleus. Unprocessed RNA is called precursor mRNA or pre-mRNA to distinguish it from mature mRNA.
Once about 20-40 ribonucleotides have been joined together by RNA polymerase, a group of enzymes adds a “cap” to the 5’ end of the growing transcript. In this process, a 5’ phosphate is replaced by modified guanosine that has a methyl group attached to it (7-Methyl guanosine). This 5’ cap helps the...
Pre-mRNA Processing02:01

Pre-mRNA Processing

In eukaryotic cells, transcripts made by RNA polymerase are modified and processed before exiting the nucleus. Unprocessed RNA is called precursor mRNA or pre-mRNA to distinguish it from mature mRNA.
Once about 20-40 ribonucleotides have been joined together by RNA polymerase, a group of enzymes adds a “cap” to the 5’ end of the growing transcript. In this process, a 5’ phosphate is replaced by modified guanosine that has a methyl group attached to it (7-Methyl guanosine). This 5’ cap helps the...
Ribosomal RNA Synthesis02:53

Ribosomal RNA Synthesis

Ribosome synthesis is a highly complex and coordinated process involving more than 200 assembly factors. The synthesis and processing of ribosomal components occurs not only in the nucleolus but also in the nucleoplasm and the cytoplasm of eukaryotic cells.
Ribosome biogenesis begins with the synthesis of 5S and 45S pre-rRNAs by distinct RNA polymerases. The primary transcripts are extensively processed and modified before they are bound and folded by ribosomal proteins and assembly factors,...
Proteins: From Genes to Degradation02:11

Proteins: From Genes to Degradation

Within a biological system, the DNA encodes the RNA, and the nucleotide sequence in the RNA further defines the amino acid sequence in the protein. This is referred to as “The Central Dogma of Molecular Biology” - a term coined by Francis Crick.  Central dogma is a firm principle in biology that defines the flow of genetic information within any life form. The two fundamental steps in central dogma are - transcription and translation.
Transcription is the synthesis of RNA molecules by RNA...
Pre-mRNA Processing: Modification of pre-mRNA Ends01:35

Pre-mRNA Processing: Modification of pre-mRNA Ends

In eukaryotic cells, transcripts made by RNA polymerase are modified and processed before exiting the nucleus. Unprocessed RNA is called precursor mRNA or pre-mRNA to distinguish it from mature mRNA.
Once about 20-40 ribonucleotides have been joined together by RNA polymerase, a group of enzymes adds a cap to the 5' end of the growing transcript. In this process, a 5' phosphate is replaced by modified guanosine that has a methyl group attached (7-methyl guanosine). This 5' cap helps the cell...
Ribosomal RNA Synthesis02:53

Ribosomal RNA Synthesis

Ribosome synthesis is a highly complex and coordinated process involving more than 200 assembly factors. The synthesis and processing of ribosomal components occurs not only in the nucleolus but also in the nucleoplasm and the cytoplasm of eukaryotic cells.
Ribosome biogenesis begins with the synthesis of 5S and 45S pre-rRNAs by distinct RNA polymerases. The primary transcripts are extensively processed and modified before they are bound and folded by ribosomal proteins and assembly factors,...

こちらも読む

関連記事

共著者、ジャーナル、引用グラフによってこの研究に関連する記事。

並び替え
Same author

Alveolar soft part sarcoma--reciprocal translocation between chromosome 17q25 and Xp11. Report of a case with metastases at presentation and review of the literature.

Acta orthopaedica Belgica·2003
Same author

Age dependent aneuploidy and telomere length of the human vascular endothelium.

Atherosclerosis·2001
Same author

Sex chromosome mosaicism in gonads of a fetus with cystic hygroma and deletion of the short arm of Y chromosome including loss of SRY.

American journal of medical genetics·2001
Same author

Loss of chromosome 13 in cultured human vascular endothelial cells.

Experimental cell research·2000
Same author

Telomere attrition of the human abdominal aorta: relationships with age and atherosclerosis.

Atherosclerosis·2000
Same author

Telomeres and essential hypertension.

American journal of hypertension·1999

関連する実験動画

Updated: Jul 23, 2026

Transcriptome Analysis of Single Cells
07:27

Transcriptome Analysis of Single Cells

Published on: April 25, 2011

グロービンRNA前駆分子:赤色素細胞における生合成とプロセス

R N Bastos, H Aviv

    Cell
    |July 1, 1977
    PubMed
    まとめ

    研究者は,パルス追跡技術を使用して赤血球のグロービンmRNA生成を研究した. 核内のより大きな前駆体分子を特定し,グロービンメッセンジャーRNA生物合成の経路を明らかにした.

    科学分野:

    • 分子生物学は分子生物学である.
    • 細胞生物学 細胞生物学
    • バイオケミストリー バイオケミストリー

    背景:

    • グロービンmRNAは赤血球の機能に不可欠である.
    • mRNAバイオシンセシスの理解は,細胞のプロセスにとって極めて重要です.

    研究 の 目的:

    • 染色体細胞におけるグロービンmRNAの生物合成経路を調査する.
    • グロービンのmRNA形成に関与する前駆者の分子を特定する.

    主な方法:

    • ラベル付けされたRNAと補完的なDNAのハイブリッド化.
    • パルス追跡技術を利用した.
    • エリソイド細胞核と細胞プラズマのRNA種を分析した.

    主要な成果:

    • グロビンmRNAと配列を共有する3つの核RNA種が検出されました.
    • 2つのより大きな前駆体:a 27S (約. 5000ヌクレオチド) と15S (約15S) を含んでいる. 1500ヌクレオチド) の分子である.
    • 27S種にはポリ (A) 配列が欠けていたが,15S種と10S種には含まれていた. 27S前駆体は急速に分解され,10S細胞質mRNAは蓄積された.

    結論:

    さらに関連する動画

    Isolation and Characterization of RNA-Containing Exosomes
    09:43

    Isolation and Characterization of RNA-Containing Exosomes

    Published on: January 9, 2012

    Preparation of Cytoplasmic and Nuclear Long RNAs from Primary and Cultured Cells
    06:29

    Preparation of Cytoplasmic and Nuclear Long RNAs from Primary and Cultured Cells

    Published on: April 7, 2023

    関連する実験動画

    Last Updated: Jul 23, 2026

    Transcriptome Analysis of Single Cells
    07:27

    Transcriptome Analysis of Single Cells

    Published on: April 25, 2011

    Isolation and Characterization of RNA-Containing Exosomes
    09:43

    Isolation and Characterization of RNA-Containing Exosomes

    Published on: January 9, 2012

    Preparation of Cytoplasmic and Nuclear Long RNAs from Primary and Cultured Cells
    06:29

    Preparation of Cytoplasmic and Nuclear Long RNAs from Primary and Cultured Cells

    Published on: April 7, 2023

    • グロービンのmRNAバイオシンセシスのモデルを提案した.
    • この経路は,大きな27S前駆体への転写を伴うもので,その後,15S中間体への分裂が続く.
    • さらに処理すると,成熟した10SグロービンのmRNAが生成され,細胞プラズマに蓄積されます.