特殊なヌクレオソームは,C. glabrataの金属反応性プロモーターへの転写因子のアクセスを調節する
1Department of Biological Chemistry, The University of Michigan Medical School, Ann Arbor 48109-0606, USA.
Cell
|November 1, 1996
まとめ
Candida glabrataにおける銅の急速な解毒は,金属反応要素を結合するAmt1転写因子に依存しています. この結合は,核細胞構造とDNAの歪みによって促進され,金属ホメオスタシスのための迅速な遺伝子活性化を可能にします.
科学分野:
- 分子生物学は分子生物学である.
- 遺伝学 遺伝学とは
- バイオケミストリー バイオケミストリー
背景:
- 転写因子は,遺伝子調節のためのDNAプロモーター要素にアクセスする必要があります.
- 急速な転写反応は,細胞防御と恒常性のために不可欠である.
研究 の 目的:
- Candida glabrataにおける Amt1転写因子による急速な転写自己活性化のメカニズムを調査する.
- Amt1機能の調節におけるDNA構造と核子の位置づけの役割を理解する.
主な方法:
- In vivoフットプリントアッセイ
- クロマチンのマッピング実験
- DNA配列要素と核細胞の位置づけの分析
主要な成果:
- Amt1媒介の急速な転写自己活性化は,銅が誘発したDNA結合と金属反応要素 (MRE) に依存しています.
- MREと隣接するホモポリマー (dA x dT) 要素は,位置づけられたヌクレオソームに詰め込まれています.
- (dA x dT) 要素に依存する核胞子に関連したDNA歪みは,Amt1結合と転写に不可欠です.
結論:
- AMT1プロモーターは,銅に対する急速な転写反応を促進するために新しい核細胞構造を使用します.
- この特殊な構造は,金属ホメオスタシスと,Candida glabrataの解毒メカニズムを結びつけている.
- 核細胞内の局所的なDNA歪みは,転写因子結合と急速な遺伝子活性化のための重要な規制機能です.
関連する概念動画
02:36RNA Polymerase II Accessory Proteins
Proteins that regulate transcription can do so either via direct contact with RNA Polymerase or through indirect interactions facilitated by adaptors, mediators, histone-modifying proteins, and nucleosome remodelers. Direct interactions to activate transcription is seen in bacteria as well as in some eukaryotic genes. In these cases, upstream activation sequences are adjacent to the promoters, and the activator proteins interact directly with the transcriptional machinery. For example, in...
01:58Prokaryotic Transcriptional Activators and Repressors
The organization of prokaryotic genes in their genome is notably different from that of eukaryotes. Prokaryotic genes are organized, such that the genes for proteins involved in the same biochemical process or function are located together in groups. This group of genes, along with their regulatory elements, are collectively known as an operon. The functional genes in an operon are transcribed together to give a single strand of mRNA known as polycistronic mRNA.
Transcription of prokaryotic...
Transcription of prokaryotic...
02:04Co-activators and Co-repressors
Gene transcription is regulated by the synergistic action of several proteins that form a complex at a gene regulatory site. This is observed in eukaryotes, where the regulation of gene expression is a complex process. Regulatory proteins in eukaryotes can broadly be classified into two types – regulators that bind directly to specific DNA sequences and co-regulators that associate with regulatory proteins but cannot directly bind to the DNA. These co-regulators are further divided into...
01:52Eukaryotic Transcription Inhibitors
Certain biochemical processes, such as embryonic development and cell growth regulation, depend on the repression of specific genes. DNA binding proteins known as eukaryotic transcription inhibitors regulate the repression of gene expression in eukaryotes. The presence of these inhibitors at the required location and time in the cell is triggered by the presence of hormones and additional signals from other cells.
Eukaryotic transcription inhibitors usually contain two distinct domains, a DNA...
Eukaryotic transcription inhibitors usually contain two distinct domains, a DNA...
01:30General Transcription Factors
Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...
01:58Prokaryotic Transcriptional Activators and Repressors
The organization of prokaryotic genes in their genome is notably different from that of eukaryotes. Prokaryotic genes are organized, such that the genes for proteins involved in the same biochemical process or function are located together in groups. This group of genes, along with their regulatory elements, are collectively known as an operon. The functional genes in an operon are transcribed together to give a single strand of mRNA known as polycistronic mRNA.
Transcription of prokaryotic...
Transcription of prokaryotic...