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パッチされた腫瘍抑制遺伝子は,Sonic hedgehogの受容体候補をコードする
D M Stone1, M Hynes, M Armanini
1Department of Neuroscience, Genentech, Inc., South San Francisco, California 94080, USA.
Nature
|November 14, 1996
まとめ
ソニック・ヘッジホッグ (Shh) タンパク質は,腫瘍抑制剤であるパッチド (Ptc) に結合する. パッチドはスムージド (Smo) と相互作用し,Ptcが脊椎動物の発達におけるShh受容体として作用することを示唆しています.
科学分野:
- 発達生物学 発達生物学とは
- 分子生物学は分子生物学である.
- がん研究 がん研究
背景:
- ソニック・ヘッジホッグ (Shh) は,脊椎動物の発達,パターニング,成長を調節する重要なタンパク質です.
- パッチド (Ptc) は,基礎細胞癌に関与する腫瘍抑制タンパク質として知られています.
- Smoothened (Smo) は,発達シグナル伝達経路における重要なタンパク質です.
研究 の 目的:
- ソニック・ヘッジホッグ (Shh) とパッチド (Ptc) の相互作用を調査する.
- 脊椎動物におけるパッチ (Ptc) とスムーズ (vSmo) の関係を探求する.
- 発達中のSHH信号の分子メカニズムを解明する.
主な方法:
- タンパク質結合試験では,ShhとPtc.との間の高親近性相互作用を実証しています.
- PtcとvSmo.mo.の間の物理的な複合体の形成を示すための共免疫プレシピテーション.
- 表現分析により,様々な組織におけるvPtcとvSmoの共局所を決定する.
主要な成果:
- ソニック・ヘッジホッグ (Shh) タンパク質は,パッチド (Ptc) に高い親和性で結合する.
- 脊椎動物のPtcは,脊椎動物のスムージドホモログ (vSmo) と物理的な複合体を形成する.
- vSmoはvPtcと共表現されるが,Shh.を直接結合することはない.
結論:
- パッチド (Ptc) は,ソニック・ヘッジホッグ (Shh) の受容体として機能する.
- 脊椎動物スムージング (vSmo) は,Ptc受容体に関連したシグナリング成分です.
- これらの発見は,PtcとvSmoを含むShh信号伝導のモデルをサポートしています.
関連する概念動画
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The Notch signaling pathway is a major intracellular signaling pathway that is highly conserved over a broad spectrum of metazoan species. It stands unique from other intracellular signaling mechanisms in animals because notch protein itself acts as the receptor as well as the primary signaling molecule.
The Notch gene came into the limelight in 1914 after the discovery that its mutation in Drosophila melanogaster leads to a serrated (or "notched") wing margin phenotype. It was not until 1985...
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