フォスフォリレーションによるポリア) ポリメラーゼの細胞サイクル関連の調節
D F Colgan1, K G Murthy, C Prives
1Department of Biological Science, Columbia University, New York 10027, USA.
Nature
|November 21, 1996
まとめ
ポリア) ポリメラーゼ (PAP) は,細胞分裂中に成熟促進因子 (MPF) によってリン酸化される. この高酸化はPAPの活性を抑制し,M相細胞におけるmRNAとタンパク質合成を潜在的に減少させる.
科学分野:
- 分子生物学は分子生物学である.
- 細胞生物学 細胞生物学
- バイオケミストリー バイオケミストリー
背景:
- ユカリオットメッセンジャーRNA (mRNA) のポリアテール尾は,翻訳開始とmRNAの安定性にとって極めて重要です.
- ポリアテール合成の調節は,特に初期の発達期において,遺伝子発現の重要なステップです.
研究 の 目的:
- ポリア) ポリメラーゼ (PAP) の活性が,p34cdc2) /サイクリンB (成熟促進因子,MPF) によるリン酸化によって制御される可能性を調査する.
主な方法:
- MPFを用いたPAPのインビヴォおよびインビトロリン酸化アッセイ.
- Xenopus laevisの卵細胞と,M相で停止されたHeLa細胞の中性成熟期におけるPAPのリン酸化状態の分析.
- 精製されたPAPとミト細胞から分離されたPAPの酵素活性測定法.
主要な成果:
- PAPはMPFによってin vivoおよびin vitroでリン酸化される.
- PAPは,Xenopusの卵細胞の成熟過程で高酸化され,M相ではHeLa細胞が逮捕されます.
- MPF媒介による高酸化はPAPの活性を著しく低下させ,ミト細胞からのPAPは抑制された活性を示します.
結論:
- MPF媒介によるPAPのリン酸化は,M相中のPAPの活性を抑制する規制メカニズムとして機能する可能性がある.
- このPAPの抑制は,Mフェーズ中に観察されたポリ (A) +RNAとタンパク質合成の減少に寄与する可能性があります.
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