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Protein Complexes with Interchangeable Parts01:57

Protein Complexes with Interchangeable Parts

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Groups of proteins may form a complex where each protein in this complex has a different role in the overall execution of the complex’s function. Often some of the proteins in the complex can be replaced by a closely related variant to give a complex that contains many of the same components yet is functionally distinct.
The SCF ubiquitin ligase is a protein complex of five individual proteins. This complex attaches ubiquitin to other target proteins to mark them for degradation. In order...
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Bacterial RNA Polymerase00:43

Bacterial RNA Polymerase

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Unlike eukaryotes, bacteria use a single RNA Polymerase (RNAP) to transcribe all genes. The different subunits of bacterial RNAPhave distinct functions. The multisubunit structure of the bacterial RNAP helps the enzyme to maintain catalytic function, facilitate assembly, interact with DNA and RNA, and self-regulate its activity.
In most genes, the transcription site is a single base present upstream of the coding sequence. Though RNAP is a catalytically efficient enzyme, it does not recognize...
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Cell Specific Gene Expression01:58

Cell Specific Gene Expression

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Multicellular organisms contain a variety of structurally and functionally distinct cell types, but the DNA in all the cells originated from the same parent cells. The differences in the cells can be attributed to the differential gene expression. Liver cells, whose functions include detoxification of blood, production of bile to metabolize fats, and synthesis of proteins essential for metabolism, must express a specific set of genes to perform their functions. Gene expression also varies with...
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TGF - β Signaling Pathway01:16

TGF - β Signaling Pathway

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The TGF-β signaling pathway regulates cell growth, differentiation, adhesion, motility, and development. TGF-β ligands that induce TGF-β signaling are synthesized in their latent form. Several proteases or cell surface receptors such as integrins act upon the latent form, releasing the active ligand. There are three types of mammalian TGF-βs: (TGF-β1, TGF-β2, and TGF-β3) that bind as homodimers or heterodimers to TGF-β receptors. The TGF-β receptors...
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Activation and Inactivation of G Proteins01:22

Activation and Inactivation of G Proteins

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Heterotrimeric G proteins are guanine nucleotide-binding proteins. As the name suggests, heterotrimeric G proteins are composed of three subunits: alpha, beta, and gamma. They remain GDP-bound or GTP-bound inside the cells and switch between inactive/active states. The Gα subunit possesses the nucleotide-binding pocket that binds guanine nucleotides and switches between GDP or GTP-bound states. In contrast, the Gꞵ and Gγ subunits are always bound together with high...
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Calmodulin-dependent Signaling01:16

Calmodulin-dependent Signaling

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Calmodulin (CaM) is a calcium-binding protein in eukaryotes that controls various calcium-regulated cellular processes. It has four calcium-binding sites that bind calcium to form the calcium-calmodulin ( Ca2+-CaM) complex. GPCR stimulation increases the calcium levels in the cells that bind to CaM and induces a conformational change.
The Ca2+-CaM complex does not have enzymatic activity by itself. Instead, the complex binds downstream target proteins, including membrane proteins or enzymes,...
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関連する実験動画

Updated: Apr 30, 2026

Bimolecular Fluorescence Complementation
08:54

Bimolecular Fluorescence Complementation

Published on: April 15, 2011

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CBFβサブユニットは,CBFalpha2 (AML1) 機能に不可欠である.

Q Wang1, T Stacy, J D Miller

  • 1Department of Biochemistry, Dartmouth Medical School, Hanover, New Hampshire 03755, USA.

Cell
|November 15, 1996
PubMed
まとめ
この要約は機械生成です。

コア結合因子β (CBFβ) のサブユニットは,CBFalpha2の機能に不可欠である. CBFB遺伝子のホモジゴス変異は,CBFA2遺伝子の破壊に類似した胚の致死性および血液形成の欠陥につながる.

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An Improved Protocol to Purify and Directly Mono-Biotinylate Recombinant BDNF in a Tube for Cellular Trafficking Studies in Neurons
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Stability and Structure of Bat Major Histocompatibility Complex Class I with Heterologous β2-Microglobulin
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Stability and Structure of Bat Major Histocompatibility Complex Class I with Heterologous β2-Microglobulin

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関連する実験動画

Last Updated: Apr 30, 2026

Bimolecular Fluorescence Complementation
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An Improved Protocol to Purify and Directly Mono-Biotinylate Recombinant BDNF in a Tube for Cellular Trafficking Studies in Neurons
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Stability and Structure of Bat Major Histocompatibility Complex Class I with Heterologous β2-Microglobulin
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科学分野:

  • ヘマトポエーシス (血液形成) とは
  • 分子生物学は分子生物学である.
  • 遺伝学 遺伝学とは

背景:

  • コア結合因子β (CBFbeta) は,ヘテロジメア型コア結合因子 (CBF) の非DNA結合子単位である.
  • CBFbetaは,CBFalphaサブユニットのDNA結合親和性を強化する.
  • CBFBとCBFA2遺伝子は,ヒト急性白血病の染色体転位に頻繁に関与しています.

研究 の 目的:

  • CBFβサブユニットのインビボ機能を調査する.
  • CBFalpha2機能にCBFbetaが必要かどうかを判断する.
  • Cbfb遺伝子障害とCbfa2遺伝子障害のフェノタイプを比較する.

主な方法:

  • Cbfb遺伝子の同同胞性障害を持つマウスの世代.
  • ミュータントマウスのフェノタイプ分析,胚死亡率の評価,中枢神経系出血,胎児肝臓出血を含む.

主要な成果:

  • Cbfb遺伝子のホモジゴス変異は,妊娠中期に胚の致死性をもたらしました.
  • ミュータントの胚は中枢神経系の出血を示し,胎児の肝臓の血液形成を阻害した.
  • 観察されたフェノタイプは,同卵性Cbfa2 (AML1) 遺伝子障害のフェノタイプと同一であった.

結論:

  • CBFβサブユニットは,CBFalpha2の in vivo 機能に不可欠である.
  • CBFβは胚の発達と血液形成において重要な役割を果たします.
  • これらの発見は,急性白血病の予防におけるCBFβ-CBFalpha2複合体の重要性を強調しています.