トランスクリプトは,RNAポリメラーゼのプロセシビティと延長率を増加させる
R A King1, S Banik-Maiti, D J Jin
1Laboratory of Molecular Genetics, National Institute of Child Health and Human Development, National Institute of Health, Bethesda, Maryland 20892, USA.
Cell
|November 29, 1996
まとめ
HK022ファージは,抗終結部位 (プット部位) で特定のRNA構造を用いて,遺伝子発現を制御する. このプロセスは,余分なタンパク質を必要とせずに転写の読み込みと延長を強化し,RNA自体がポリメラーゼの活動を調節することを示唆しています.
科学分野:
- 分子生物学は分子生物学である.
- ウイルス学 ウイルス学 ウイルス学
- 遺伝子規制 遺伝子規制
背景:
- ラムドイドファグHK022は,トランスクリプション終止信号を克服するために,シス作用のアンチターミネーションサイト (プットサイト) を利用します.
- アンチターミネーションはファグの遺伝子発現に不可欠ですが,しばしば複雑なタンパク質機構が含まれます.
- HK022アンチターミネーションの正確なメカニズムとそのRNA構造への依存は完全に解明されていません.
研究 の 目的:
- フェーグHK022のプット部位によって媒介されるアンチターミネーションのメカニズムを調査する.
- この過程におけるRNA構成と細胞タンパク質の役割を決定する.
- プットサイトがRNAポリメラーゼの延長と一時停止に与える影響を特徴付けるために.
主な方法:
- 予測されたRNA幹形成に影響を与えるプット変異の分析と,その抗終結への影響.
- 浄化されたRNAポリメラーゼ,テンプレートDNA,および基板を用いたインビトロ転写アッセイ.
- RNAポリメラーゼβ'サブユニットのサイト指向型変異を,抗終末化におけるその役割を評価する.
主要な成果:
- アンチターミネーション活動は,プットトランスクリプト内の特定のRNA幹構造の形成に厳密に依存しています.
- 浄化されたシステムでは,ターミネーターのプット依存の読み込みと伸び率の増加が観察され,追加のタンパク質の必要がないことを示しました.
- ベータ'サブユニットの亜鉛指領域における突然変異は,転写に対するプット媒介効果を廃止した.
結論:
- HK022アンチターミネーションは,補助タンパク質を必要としない単純なRNAベースのメカニズムです.
- プットトランスクリプトの構成は,読み込みと停止の抑制を含むRNAポリメラーゼの延長特性を直接調節します.
- このRNA中心のメカニズムは,他の既知のアンチターミネーション経路に対して明確な代替案を提供します.
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関連する概念動画
01:10Transcription
Overview
Transcription is the process of synthesizing RNA from a DNA sequence by RNA polymerase. It is the first step in producing a protein from a gene sequence. Additionally, many other proteins and regulatory sequences are involved in the proper synthesis of messenger RNA (mRNA). Regulation of transcription is responsible for the differentiation of all the different types of cells and often for the proper cellular response to environmental signals.
Transcription Can Produce Different Kinds...
Transcription is the process of synthesizing RNA from a DNA sequence by RNA polymerase. It is the first step in producing a protein from a gene sequence. Additionally, many other proteins and regulatory sequences are involved in the proper synthesis of messenger RNA (mRNA). Regulation of transcription is responsible for the differentiation of all the different types of cells and often for the proper cellular response to environmental signals.
Transcription Can Produce Different Kinds...
02:36RNA Polymerase II Accessory Proteins
Proteins that regulate transcription can do so either via direct contact with RNA Polymerase or through indirect interactions facilitated by adaptors, mediators, histone-modifying proteins, and nucleosome remodelers. Direct interactions to activate transcription is seen in bacteria as well as in some eukaryotic genes. In these cases, upstream activation sequences are adjacent to the promoters, and the activator proteins interact directly with the transcriptional machinery. For example, in...
02:35Transcription Elongation Factors
Transcription elongation is a dynamic process that alters depending upon the sequence heterogeneity of the DNA being transcribed. Hence, it is not surprising that the elongation complex's composition also varies along the way while transcribing a gene.
The transcription elongation is regulated via pausing of RNA polymerase on several occasions during transcription. In bacteria, these halts are necessary because the transcription of DNA into mRNA is coupled to the translation of that mRNA into a...
The transcription elongation is regulated via pausing of RNA polymerase on several occasions during transcription. In bacteria, these halts are necessary because the transcription of DNA into mRNA is coupled to the translation of that mRNA into a...
01:17Transcription
Transcription is the synthesis of RNA from a DNA sequence by RNA polymerase. It is the first step in producing a protein from a gene sequence. Additionally, many other proteins and regulatory sequences are involved in correctly synthesizing messenger RNA (mRNA). Transcriptional regulation is responsible for the differentiation of different types of cells and often for the proper cellular response to environmental signals.
Transcription Can Produce Different Kinds of RNA Molecules
In eukaryotes,...
Transcription Can Produce Different Kinds of RNA Molecules
In eukaryotes,...
02:36RNA Polymerase II Accessory Proteins
Proteins that regulate transcription can do so either via direct contact with RNA Polymerase or through indirect interactions facilitated by adaptors, mediators, histone-modifying proteins, and nucleosome remodelers. Direct interactions to activate transcription is seen in bacteria as well as in some eukaryotic genes. In these cases, upstream activation sequences are adjacent to the promoters, and the activator proteins interact directly with the transcriptional machinery. For example, in...
02:35Transcription Elongation Factors
Transcription elongation is a dynamic process that alters depending upon the sequence heterogeneity of the DNA being transcribed. Hence, it is not surprising that the elongation complex's composition also varies along the way while transcribing a gene.
The transcription elongation is regulated via pausing of RNA polymerase on several occasions during transcription. In bacteria, these halts are necessary because the transcription of DNA into mRNA is coupled to the translation of that mRNA into a...
The transcription elongation is regulated via pausing of RNA polymerase on several occasions during transcription. In bacteria, these halts are necessary because the transcription of DNA into mRNA is coupled to the translation of that mRNA into a...