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Updated: Aug 12, 2026

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Nanomanipulation of Single RNA Molecules by Optical Tweezers
Published on: August 20, 2014
触媒RNA中間物の構造を捕捉する:ハンマーヘッドリボ酵素
W G Scott1, J B Murray, J R Arnold
1MRC Laboratory of Molecular Biology, Hills Road, Cambridge CB2 2QH, England.
まとめ
ハンマーヘッドリボジームの結晶構造が解明され,その活性折れが明らかになった. 二重金属イオンは,活性部位で形状の変化を誘導し,自己分裂を促進します.
科学分野:
- バイオケミストリー バイオケミストリー
- 構造生物学 構造生物学とは
- RNAカタリシスによるRNAカタリシス
背景:
- ハンマーヘッドリボ酵素は,自己分裂を可能にする重要なRNA酵素である.
- 構造と機能の関係を理解することは,RNA生物学にとって重要なことです.
研究 の 目的:
- 無修正のハンマーヘッドリボ酵素の結晶構造を決定する.
- カタリシス中に二価金属イオンによって誘発される形状の変化を調査する.
主な方法:
- ハンマーヘッドリボジーム構造を解明するために,X線結晶学を用いた.
- 活性pHでの冷凍トラップは,触媒中間物質を捕獲した.
主要な成果:
- 改変されていないリボ酵素の生物学的に活性な折り合いを明らかにしました.
- 二対子カチオンは,裂解部位のリン酸と結合し,コンフォーメーション中間物質を形成することが示された.
- 形状の変化は活性部位に局限し,移行状態に近づいています.
結論:
- ハンマーヘッドリボ酵素は,さまざまな改変において保存された折り畳みを採用します.
- 金属イオンの結合とそれに続く構成の変化は,ハンマーヘッドリボジーム触媒にとって重要である.
関連する概念動画
RNA Structure
Overview
The basic structure of RNA consists of a five-carbon sugar and one of four nitrogenous bases. Although most RNA is single-stranded, it can form complex secondary and tertiary structures. Such structures play essential roles in the regulation of transcription and translation.
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA): messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three RNA types consist of a...
The basic structure of RNA consists of a five-carbon sugar and one of four nitrogenous bases. Although most RNA is single-stranded, it can form complex secondary and tertiary structures. Such structures play essential roles in the regulation of transcription and translation.
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA): messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three RNA types consist of a...
RNA Structure
Overview
The basic structure of RNA consists of a five-carbon sugar and one of four nitrogenous bases. Although most RNA is single-stranded, it can form complex secondary and tertiary structures. Such structures play essential roles in the regulation of transcription and translation.
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA): messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three RNA types consist of a...
The basic structure of RNA consists of a five-carbon sugar and one of four nitrogenous bases. Although most RNA is single-stranded, it can form complex secondary and tertiary structures. Such structures play essential roles in the regulation of transcription and translation.
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA): messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three RNA types consist of a...
Ribozymes
The term ribozyme is used for RNA that can act as an enzyme. Ribozymes are mainly found in selected viruses, bacteria, plant organelles, and lower eukaryotes. Ribozymes were first discovered in 1982 when Tom Cech’s laboratory observed Group I introns acting as enzymes. This was shortly followed by the discovery of another ribozyme, Ribonulcease P, by Sid Altman’s laboratory. Both Cech and Altman received the Nobel Prize in chemistry in 1989 for their work on ribozymes.
Ribozymes can be...
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ATP synthase or ATPase is among the most conserved proteins found in bacteria, mammals, and plants. This enzyme can catalyze a forward reaction in response to the electrochemical gradient, producing ATP from ADP and inorganic phosphate. ATP synthase can also work in a reverse direction by hydrolyzing ATP and generating an electrochemical gradient. Different forms of ATP synthases have evolved special features to meet the specific demands of the cell. Based on their specific feature, ATP...
Ribozymes
The term ribozyme is used for RNA that can act as an enzyme. Ribozymes are mainly found in selected viruses, bacteria, plant organelles, and lower eukaryotes. Ribozymes were first discovered in 1982 when Tom Cech’s laboratory observed Group I introns acting as enzymes. This was shortly followed by the discovery of another ribozyme, Ribonulcease P, by Sid Altman’s laboratory. Both Cech and Altman received the Nobel Prize in chemistry in 1989 for their work on ribozymes.
Ribozymes can be...
Ribozymes can be...
RNA Structure
The basic structure of RNA consists of a string of ribonucleotides attached by phosphodiester bonds. Although most RNA is single-stranded, it can form complex secondary and tertiary structures. Such structures play essential roles in the regulation of transcription and translation.
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA) involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three...
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA) involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three...

