TAF (II) は,ドロソフィラの胚における転写の活性化を媒介する

F Sauer¹, D A Wassarman, G M Rubin

¹Howard Hughes Medical Institute, Department of Molecular and Cell Biology, University of California, Berkeley 94720-3204, USA.

Cell

|December 27, 1996

まとめ

TAF遺伝子の変異は,ビコイド標的の転写を損なう. TAFは共同活性化剤として作用し,発達の過程で転写調節のための遺伝子特異的な信号を受け取ります.

科学分野:

分子生物学は分子生物学である.
発達生物学発達生物学とは
遺伝学遺伝学とは

背景:

転写因子とコアクティベーターは,遺伝子調節に不可欠です.
TAF (II) 60とTAF (II) 110は,トランスクリプション・マシンの保存された構成要素である.
ビコイドは,胚における重要な発達調節剤である.

研究の目的:

転写におけるTAF (II) 60とTAF (II) 110のインビボの役割を調査する.
TAFが特定の転写活性化剤の共同活性化剤として機能するかどうかを判断する.
開発における基礎転写の研究のための遺伝子システムの使用を探求する.

主な方法:

遺伝子検査システム in vivo を利用する.
TAF遺伝子の変異を分析した (TAF (II) 60とTAF (II) 110)).
ビコイド依存遺伝子転写への影響を評価する.

主要な成果:

TAF (II) 60とTAF (II) 110の変異は,ビコイド標的遺伝子の転写を低下させる.
特定のアクティベーター-TAFの相互作用は,単純にシネージー的に転写を強化することが示されました.
In vivoの証拠は,TAFが転写信号の共同活性化剤として作用することを支持しています.

結論:

TAFは,遺伝子転写におけるコアクティベーターとして重要な役割を果たします.
特定のアクティベーター-TAF相互作用は,遺伝子発現の調節に不可欠です.
この遺伝システムは,複雑な生物における基礎転写の研究を容易にする.

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