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Yeast Signaling01:28

Yeast Signaling

Yeasts are single-celled organisms, but unlike bacteria, they are eukaryotes (cells with a nucleus). Cell signaling in yeast is similar to signaling in other eukaryotic cells. A ligand, such as a protein or a small molecule released from a yeast cell, attaches to a receptor on the cell surface. The binding stimulates second-messenger kinases to activate or inactivate transcription factors that further regulate gene expression. Many of the yeast intracellular signaling cascades have similar...
Transcription Attenuation in Prokaryotes02:42

Transcription Attenuation in Prokaryotes

Transcriptional attenuation occurs when RNA transcription is prematurely terminated due to the formation of a terminator mRNA hairpin structure.  Bacteria use these hairpins to regulate the transcription process and control the synthesis of several amino acids including histidine, lysine, threonine, and phenylalanine. Transcription attenuation takes place in the non-coding regions of mRNA.
There are several different mechanisms used to attenuate transcription. In ribosome mediated...
Ribosome Profiling02:24

Ribosome Profiling

Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique helps...
mRNA Stability and Gene Expression02:51

mRNA Stability and Gene Expression

The structure and stability of mRNA molecules regulates gene expression, as mRNAs are a key step in the pathway from gene to protein. In eukaryotes, the half-life of mRNA varies from a few minutes up to several days. mRNA stability is essential in growth and development. The absence of the proteins regulating its stability, such as tristetraprolin in mice, can cause systemic issues, including bone marrow overgrowth, inflammation, and autoimmunity.
Cis-acting Elements involved in mRNA stability
Transcription in Prokaryotes01:28

Transcription in Prokaryotes

Transcription is a highly regulated process that converts genetic information into RNA molecules. The transcription cycle is divided into three key stages: initiation, elongation, and termination, each driven by specific molecular mechanisms.Initiation of TranscriptionIn bacteria, transcription begins when the RNA polymerase core enzyme associates with a sigma factor to form a holoenzyme. For example, the E. coli sigma factor called σ70 forms a holoenzyme, which recognizes the -10 (Pribnow box)...

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Updated: Jul 8, 2026

Genome-wide Quantification of Translation in Budding Yeast by Ribosome Profiling
12:57

Genome-wide Quantification of Translation in Budding Yeast by Ribosome Profiling

Published on: December 21, 2017

イーストのトランスクリプトームの特徴

V E Velculescu1, L Zhang, W Zhou

  • 1Program in Human Genetics and Molecular Biology, The Johns Hopkins University School of Medicine, Baltimore, Maryland 21231, USA.

Cell
|January 24, 1997
PubMed
まとめ
この要約は機械生成です。

研究者は,遺伝子発現のシリアル分析を使用して,酵母遺伝子の発現をマッピングしました. この全ゲノム研究により,新しいものを含む何千もの遺伝子が明らかになり,染色体全体にわたる活性転写領域が特定されました.

さらに関連する動画

Saccharomyces cerevisiae Metabolic Labeling with 4-thiouracil and the Quantification of Newly Synthesized mRNA As a Proxy for RNA Polymerase II Activity
09:21

Saccharomyces cerevisiae Metabolic Labeling with 4-thiouracil and the Quantification of Newly Synthesized mRNA As a Proxy for RNA Polymerase II Activity

Published on: October 22, 2018

High Throughput Yeast Strain Phenotyping with Droplet-Based RNA Sequencing
07:55

High Throughput Yeast Strain Phenotyping with Droplet-Based RNA Sequencing

Published on: May 21, 2020

関連する実験動画

Last Updated: Jul 8, 2026

Genome-wide Quantification of Translation in Budding Yeast by Ribosome Profiling
12:57

Genome-wide Quantification of Translation in Budding Yeast by Ribosome Profiling

Published on: December 21, 2017

Saccharomyces cerevisiae Metabolic Labeling with 4-thiouracil and the Quantification of Newly Synthesized mRNA As a Proxy for RNA Polymerase II Activity
09:21

Saccharomyces cerevisiae Metabolic Labeling with 4-thiouracil and the Quantification of Newly Synthesized mRNA As a Proxy for RNA Polymerase II Activity

Published on: October 22, 2018

High Throughput Yeast Strain Phenotyping with Droplet-Based RNA Sequencing
07:55

High Throughput Yeast Strain Phenotyping with Droplet-Based RNA Sequencing

Published on: May 21, 2020

科学分野:

  • ゲノミクスゲノミクスとは
  • 分子生物学は分子生物学である.
  • イースト遺伝学 イースト遺伝学

背景:

  • イーストのトランスクリプトームを理解することは,真核生物の遺伝子調節を解読する上で極めて重要です.
  • 過去の研究では,全ゲノムにわたる包括的な発現データがなかった.
  • 遺伝子発現の連続分析 (SAGE) は,トランスクリプト分析のための高スループットメソッドを提供します.

研究 の 目的:

  • イーストゲノムにおける発現遺伝子の完全なセットを分析する.
  • 位置データと表現データを統合した染色体表現マップを生成する.
  • 以前に特徴づけられていなかった遺伝子と転写活動領域を特定する.

主な方法:

  • 酵母トランスクリプトームを分析するために,遺伝子発現の連続分析 (SAGE) を利用しました.
  • 6万以上のトランスクリプトの表現レベルを定量化しました.
  • ゲノムマッピングのための位置情報を含む統合遺伝子発現データ.

主要な成果:

  • 4,665の発現遺伝子を特定し,細胞当たり0.3から200以上のトランスクリプトを検出しました.
  • 既知の機能を持つ1981の遺伝子と,特徴づけられていない2684の遺伝子が見つかりました.
  • 染色体表現マップを生成し,転写活動の物理的領域を明らかにし,新しい遺伝子を特定しました.

結論:

  • イーストの遺伝子発現パターンに関する世界的な洞察を提供した.
  • ユカリオットにおける全ゲノム発現研究を実施する可能性を実証した.
  • SAGEが新たな遺伝子や規制領域を発見する可能性を強調した.