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In vitro Organoid Culture of Primary Mouse Colon Tumors
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Cdx2変異マウスのホメオシスと腸内腫瘍
K Chawengsaksophak1, R James, V E Hammond
1Howard Florey Institute of Experimental Physiology and Medicine, University of Melbourne, Parkville, Victoria, Australia.
Nature
|March 6, 1997
まとめ
ホメオボックス遺伝子Cdx2は,マウスの発達に不可欠です. マウスのCdx2機能の喪失は,発達障害と腸内腫瘍の発生率が高いことにつながり,腫瘍抑制におけるCdx2の役割を示唆しています.
科学分野:
- 発達生物学 発達生物学について
- 遺伝学 遺伝学とは
- 腫瘍学 腫瘍学
背景:
- ドロソフィラのホメオボックス遺伝子caudalは,体パターニングに不可欠です.
- 尾関節のマウスホモローグにはCdx1,Cdx2,Cdx4が含まれる.
研究 の 目的:
- ネズミのCdx2遺伝子の機能を研究するために,ゼロ変異を生成します.
- 胚の発達と腸内腫瘍発生におけるCdx2の役割を決定する.
主な方法:
- マウスのCdx2ヌル突然変異を生成するために,同種の再結合が使用されました.
- Cdx2ホモジゴートおよびヘテロジゴートヌル変異体のフェノタイプ分析.
- 骨格分析と腸ポリプ検査.
主要な成果:
- Cdx2同位体ゼロ変異体は,胚の発達初期 (3.5〜5.5日) に死亡する.
- Cdx2ヘテロジゴト変異体は,尾の欠陥や脊椎/肋骨の異常を含む発達異常を示しています.
- Cdx2ヘテロジゴットの90%では,Cdx2発現を欠く腫瘍細胞が多発性腸内アデノマトスポリープを発症する.
結論:
- Murine Cdx2は,正常な胚の発達に不可欠である.
- Cdx2は,腸内腫瘍の形成を防ぐのに重要な役割を果たします.
- Cdx2変異は,特定の腸内腫瘍の発症における主要なイベントであるようです.
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