関連する実験動画
Updated: Jul 18, 2026
10:10
Dissection and Immunostaining of Imaginal Discs from Drosophila melanogaster
Published on: September 20, 2014
LEF-1は,翼のない人々や頭半身麻痺の人の信号入力を調整する核因子である
1Medical Research Council, Laboratory of Molecular Biology, Cambridge, United Kingdom.
Cell
|March 21, 1997
まとめ
転写因子LEF-1はUltrabithorax遺伝子増強剤と結合し,遺伝子発現を調節する. LEF-1は,ドロソフィラの正確な発達制御のために,無翼とデカペンタプラジー信号を統合しています.
科学分野:
- 発達生物学 発達生物学とは
- 分子遺伝学 分子遺伝学
- シグナルトランスデュークション.
背景:
- 翼のない (wg) とデカペンタプレジック (dpp) の信号伝達経路は,ドロソフィラの胚の発達に不可欠である.
- これらの経路は,Ultrabithorax (Ubx) のようなホメオティック遺伝子を含む,重要な発達遺伝子の発現を調節する.
研究 の 目的:
- Ultrabithorax midgut enhancer内の最小の翼のない応答配列を特定し,特徴づけること.
- ミュアンの転写因子LEF-1が翼のないシグナル伝達を媒介し,Ubx発現を調節する役割を調査する.
- LEF-1が強化器の活動のために複数のシグナル入力を統合する方法を理解する.
主な方法:
- Ubx midgut enhancerにおける最小限の翼のない応答配列の識別.
- このシーケンスへのLEF-1結合の解析は,アーマディロとの三元複合体において行われました.
- LEF-1による転写刺激を評価するために,安定変換アッセイを評価します.
- 発達のフェノタイプを観察するために,LEF-1の過剰発現の研究.
- トランスクリプション活動のDPPシグナル伝達に対するLEF-1の依存性の調査.
主要な成果:
- Ultrabithorax増強剤における最小限の無翼反応シーケンスが定義されました.
- ネズミの転写因子LEF-1は,この配列に結合し,アーマディロとの三元複合体を形成します.
- LEF-1による転写刺激はアーマディロに依存し,デカペンタペリジック反応配列を必要とします.
- LEF-1の過剰発現は,翼のない超刺激現象型を模倣し,翼のないシグナル伝達の必要性を回避します.
結論:
- LEF-1は,Ultrabithorax発現を調節する際,翼のないシグナル伝達とデカペンタプラジーシグナル伝達の重要な媒介者として作用する.
- LEF-1は複数の位置信号を統合して,強化器の活動を調整します.
- LEF-1は,ドロソフィラの発達中にマルチタンパク質増強剤複合体の組み立てにおいて建築的役割を果たします.
関連する概念動画
02:54Canonical Wnt Signaling Pathway
The gene encoding the main signaling molecules of the Wnt signaling pathways (the Wnt proteins) was discovered almost four decades ago by Nüsslein-Volhard and Wieschaus. They identified and originally named the gene "wingless" (wg) after a phenotype discovered during their landmark genetic screen in Drosophila for body pattern defects. At around the same time, another researcher named Harold Varmus found that a murine tumor virus activates the mammalian wg homolog, Int-1, which results in tumor...
02:33Hedgehog Signaling Pathway
The Hedgehog gene (Hh) was first discovered due to its control of the growth of disorganized, hair-like bristles phenotype in Drosophila, much like hedgehog spines. Hh plays a crucial role in the development of organs and the maintenance of homeostasis in both invertebrates and vertebrates. However, while Drosophila has only one Hh protein, mammals have multiple functional Hedgehog proteins - Sonic (Shh), Desert (Dhh), and Indian Hedgehog (Ihh). All of these homologous proteins have adapted to...
01:41Non-Canonical Wnt Signaling Pathways
Wnt is a zygotic effect gene that is expressed during very early embryonic development. It regulates various processes in animals starting from early development through the adult stage, such as organogenesis in the embryo and maintenance of neuronal and blood stem cells. Wnt proteins can induce a wide variety of intracellular pathways depending upon the specific abilities of different Wnt ligands to form a complex with shared and cognate receptors in the presence of different co-receptors. The...
02:54Canonical Wnt Signaling Pathway
The gene encoding the main signaling molecules of the Wnt signaling pathways (the Wnt proteins) was discovered almost four decades ago by Nüsslein-Volhard and Wieschaus. They identified and originally named the gene "wingless" (wg) after a phenotype discovered during their landmark genetic screen in Drosophila for body pattern defects. At around the same time, another researcher named Harold Varmus found that a murine tumor virus activates the mammalian wg homolog, Int-1, which results in tumor...
01:41Non-Canonical Wnt Signaling Pathways
Wnt is a zygotic effect gene that is expressed during very early embryonic development. It regulates various processes in animals starting from early development through the adult stage, such as organogenesis in the embryo and maintenance of neuronal and blood stem cells. Wnt proteins can induce a wide variety of intracellular pathways depending upon the specific abilities of different Wnt ligands to form a complex with shared and cognate receptors in the presence of different co-receptors. The...
02:33Hedgehog Signaling Pathway
The Hedgehog gene (Hh) was first discovered due to its control of the growth of disorganized, hair-like bristles phenotype in Drosophila, much like hedgehog spines. Hh plays a crucial role in the development of organs and the maintenance of homeostasis in both invertebrates and vertebrates. However, while Drosophila has only one Hh protein, mammals have multiple functional Hedgehog proteins - Sonic (Shh), Desert (Dhh), and Indian Hedgehog (Ihh). All of these homologous proteins have adapted to...