まとめ
研究者は,制限性エンドヌクレアゼとDNAハイブリデーションを使用して,ウサギのβ-グロービン遺伝子をマッピングしました. この分析により,ゲノムごとに単一の遺伝子コピーが明らかになり,その転写方向が決定されました.
科学分野:
- 分子生物学は分子生物学である.
- 遺伝学 遺伝学とは
- ゲノミクスゲノミクスとは
背景:
- グロービン遺伝子の構造と組織を理解することは,ヘモグロビノパシーの研究に不可欠です.
- ウサギのβ-グロービンは,真核生物の遺伝子調節と構造を調査するためのモデルシステムとして機能しています.
研究 の 目的:
- ウサギのベータ・グロービン遺伝子場所の物理的な地図を構築する.
- ウサギのゲノムにおけるβ-グロービン遺伝子の複製数を決定する.
- ベータ・グロービン遺伝子の転写の方向を確かめるために.
主な方法:
- ウサギの肝臓DNAは,制限性エンドヌクレアゼ (Eco RI, Pst I, Kpn I) で消化されました.
- DNAの断片はアガロースゲル電泳により分離され,ニトロセルロースフィルター (ブラッティング) に転送されました.
- ハイブリダイゼーションは,ウサギのベータ・グロービン補完DNA (cDNA) 探査機 (PbetaG1) を含むラベル付きのプラズミッドを使用して実施されました.
主要な成果:
- 限られた数のウサギのDNA断片は,β-グロービンのcDNAプローブで強くハイブリッド化しました.
- Eco RIの消化により2つの断片が得られ,Pst IとKpn Iはそれぞれ1つの断片が得られ,遺伝子の相対的な制限部位を示した.
- 二重消化実験では,断片の順番を決めることができ,ベータ・グロービン遺伝子周辺の制限部位の物理的な地図を確立しました.
結論:
- ウサギのゲノムは,ハプロイドゲノムごとにβ-グロービン遺伝子の単一のコピーを含んでいます.
- 制限酵素の分裂部位を含むβ-グロービン遺伝子ローカスの物理的な地図が作成されました.
- ウサギのβ-グロービン遺伝子の転写の方向は,構築された物理マップ内で決定されました.
関連する概念動画
Karyotyping
49.3K
Overview
49.3K
Rab Proteins
4.0K
Rab proteins constitute the largest family of monomeric GTPases, of which 70 members are present in humans. Rab proteins and their effectors regulate consecutive stages of vesicle transport such as vesicle transport, docking, and fusion to the correct recipient membrane.
Rab proteins switch between a cytosolic, GDP-bound inactive state and a membrane-anchored, GTP-bound active state. By themselves, Rabs show slow rates of GDP/GTP exchange and GTP hydrolysis. Thus, Rab proteins are considered...
Rab proteins switch between a cytosolic, GDP-bound inactive state and a membrane-anchored, GTP-bound active state. By themselves, Rabs show slow rates of GDP/GTP exchange and GTP hydrolysis. Thus, Rab proteins are considered...
4.0K
Reporter Genes
11.4K
Reporter genes are a type of protein-coding gene that are often tagged to a gene of interest. Once inside a target cell, reporter genes usually produce visually identifiable characteristics like fluorescence and luminescence when expressed along with the gene of interest. Thus, reporter genes “report” the presence or absence of genes of interest in an organism, determine the gene expression pattern, or track the physical location of a DNA segment or protein in the cell.
11.4K
Ribosome Profiling
3.2K
Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
3.2K
General Transcription Factors
5.9K
Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...
5.9K
Structure of a Gene
12.7K
A gene is the fundamental unit of heredity. Every individual has two copies of each gene, one inherited from each parent. Although most people contain the same genes, there is a small fraction that is slightly different amongst people. A gene with a small difference in its sequence of DNA bases forms different alleles, contributing to different phenotypes.
However, only 1% of the DNA is composed of genes that encode proteins; the rest, 99% is non-coding DNA. This non-coding DNA performs...
However, only 1% of the DNA is composed of genes that encode proteins; the rest, 99% is non-coding DNA. This non-coding DNA performs...
12.7K


