mSin3コアプレッサーと関連したヒストン脱エチラゼは,マッドの転写抑制を媒介する
C D Laherty1, W M Yang, J M Sun
1Division of Basic Sciences, Fred Hutchinson Cancer Research Center, Seattle, Washington 98104, USA.
Cell
|May 2, 1997
まとめ
Mad-Maxタンパク質は,mSin3-HDAC複合体を採用して,転写を抑制する. この複合体はヒストンを脱酸化し,クロマチンの構造を変え,遺伝子発現を阻害し,トリコスタチンAによって抑制されるプロセスである.
科学分野:
- 分子生物学は分子生物学である.
- エピジェネティクス エピジェネティクス
- 遺伝子規制 遺伝子規制
背景:
- マッドマックスヘテロジメは,転写抑制を媒介する.
- コアプレッサーmSin3A/Bは,Madタンパク質と相互作用する.
- ヒストン脱酸化酵素 (HDACs) は,染色体調節において決定的な役割を果たします.
研究 の 目的:
- mSin3A,HDAC1/2,およびMadタンパク質の相互作用を調査する.
- マッドマックス媒介による転写抑制のメカニズムを解明する.
主な方法:
- インビヴォ関連研究.
- タンパク質複合体の形成の分析.
- ヒストン脱酸化酵素の活性度の評価.
- トリコスタチンAを用いた抑制研究
主要な成果:
- mSin3AとHDAC1/2は,体内で関連していることが判明しました.
- mSin3AへのHDAC2結合は,特定の保存領域に依存しています.
- Mad1はmSin3とHDAC2との複合体を形成し,ヒストン脱酸化酵素活性を持つ.
- トリコスタチンAは,マッド媒介抑制を阻害しました.
結論:
- マッドマックスは,mSin3-HDACコアプレッサー複合体を採用することで機能します.
- この複合体は核細胞ヒストンを脱酸化します.
- 脱酸化によって誘発されるクロマチンの構造の変化は,転写阻害につながる.
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The enzyme histone acetyltransferase adds acetyl group to the histones. Another enzyme, histone deacetylase,...
Acetylation
The enzyme histone acetyltransferase adds acetyl group to the histones. Another enzyme, histone deacetylase,...