ヒストン脱エチラゼとSAP18は,新しいポリペプチドであり,ヒトのSin3複合体の構成要素である
Y Zhang1, R Iratni, H Erdjument-Bromage
1Howard Hughes Medical Institute, Department of Biochemistry, University of Medicine and Dentistry of New Jersey, Robert Wood Johnson Medical School, Piscataway 08854, USA.
Cell
|May 2, 1997
まとめ
遺伝子発現に関与する哺乳類のSin3 (mSin3) 複合体は,ヒストン脱酸化酵素 (HDAC1/HDAC2) と相互作用する. 新しいタンパク質SAP18とSAP30は,この複合体の一部として特定され,SAP18はmSin3と直接相互作用して転写を抑制する.
科学分野:
- 分子生物学は分子生物学である.
- 遺伝子規制 遺伝子規制
- エピジェネティクス エピジェネティクス
背景:
- ヒストンの改変は,遺伝子発現の調節に極めて重要です.
- 哺乳類のSin3 (mSin3) は,これらのプロセスに関与する転写抑制剤である.
研究 の 目的:
- mSin3を含む複合体の組成を調べるために.
- mSin3.3に関連した新しいタンパク質を特定する.
- 転写抑制におけるこれらの関連タンパク質の役割を明らかにする.
主な方法:
- 複合成分を特定するための共免疫プレシピテーション.
- cDNAの分離と特徴づけ.
- In vivoトランスクリプションアッセイ.
主要な成果:
- mSin3複合体はヒストン脱酸化酵素HDAC1およびHDAC2.2を含む.
- 2つの新しいmSin3関連ポリペプチド,SAP18とSAP30が特定されました.
- SAP18はmSin3と直接相互作用し,プロモーターに結合すると転写抑制を媒介する.
結論:
- mSin3複合体は,表遺伝的遺伝子調節に関与するマルチタンパク質集合体である.
- SAP18は,mSin3複合体の新しい成分で,転写抑制に寄与する.
- mSin3複合体の理解は,遺伝子サイレンシングのメカニズムについての洞察を提供します.
関連する概念動画
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Acetylation
The enzyme histone acetyltransferase adds acetyl group to the histones. Another enzyme, histone deacetylase,...
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