膀ドッキングタンパク質p115は,シス-ゴルギ行列タンパク質であるGM130をミトーシス調節された方法で結合する
N Nakamura1, M Lowe, T P Levine
1Cell Biology Laboratory, Imperial Cancer Research Fund, London, United Kingdom.
Cell
|May 2, 1997
まとめ
GM130は,シス-ゴルギのマトリックスタンパク質であり,p115に結合し,p115は,膀ドッキングを媒介するタンパク質である. この相互作用はミトスリン酸化によって調節され,細胞分裂中のゴルギ分裂を説明する.
科学分野:
- 細胞生物学 細胞生物学
- 分子生物学は分子生物学である.
- ゴルジ装置の機能 ゴルジ装置の機能
背景:
- 膀輸送と膜ドッキングは,細胞組織にとって極めて重要です.
- タンパク質p115は,トランスポートベジクルが標的膜にドッキングするのを媒介することに関与しています.
- この過程におけるシス-ゴルギ行列タンパク質GM130の役割は完全に理解されていません.
研究 の 目的:
- GM130とp115.5の相互作用を調査する.
- ミトーシス中のゴルギ分裂の基礎となる分子メカニズムを解明する.
主な方法:
- タンパク質の相互作用を研究するための共免疫プレシピテーション.
- 拘束ドメインをマッピングするための削除分析.
- ペプチド抑制アッセイ. ペプチド抑制アッセイ.
- ミトスの断片からゴルギの再編成の分析.
主要な成果:
- GM130は特にp115と相互作用し,GM130が潜在的な膜ドッキングサイトであることを示唆しています.
- GM130のN端はp115と結合し,C端はゴルギ膜と結合する.
- GM130のミトーシスリン酸化は,p115.5への結合を阻害する.
- リン酸化GM130ペプチドはNSF依存のゴルギ再構成を阻害するが,p97依存の再構成は阻害しない.
結論:
- GM130はp115のドッキングサイトとして機能し,膀輸送をゴルギと結びつける.
- GM130のミトーシスリン酸化はp115結合を阻害し,ゴルギ分裂に寄与する.
- これらの発見は,ミトーシス中のCOPI媒介のゴルギ分裂の分子基礎を提供する.
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