翻訳と時間固有のリン酸化の代替的開始により,必須のクロックタンパク質の複数の形態が得られます
N Y Garceau1, Y Liu, J J Loros
1Department of Biochemistry, Dartmouth Medical School, Hanover, New Hampshire 03755-3844, USA.
Cell
|May 2, 1997
まとめ
周波数 (FRQ) 遺伝子
科学分野:
- 分子生物学は分子生物学である.
- クロノバイオロジー クロノバイオロジー
背景:
- 周波数 (frq) 遺伝子は,神経胞の昼間振動器の中心的なものです.
- シルカディアンリズムは,転写/翻訳フィードバックループによって調節されます.
研究 の 目的:
- FRQの複雑な転写後の調節を調査する.
- FRQ合成とリン酸化の時間的動態を解明する.
主な方法:
- 代替翻訳イニシアチブによって生成されたFRQタンパク質イソフォームの分析.
- シルカディアンサイクルを通してFRQのリン酸化パターンのモニタリング.
主要な成果:
- 2つのFRQタンパク質形態が,代替翻訳開始から生じる.
- FRQは漸進的なリン酸化を経て,一時的に異なる製品を生み出します.
- frqトランスクリプトに対する翻訳的調節とFRQフィードバックは急速に発生し,その後,より遅い翻訳後の調節が起こります.
結論:
- 翻訳制御とリン酸化を含む複合的なポストトランスクリプションの調節は,ニューロスポラの昼間時計を微調整します.
- これらの規制層は,FRQの周回とfrq遺伝子の周期的な再活性化に寄与する.
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