アラビドプシスの開花時間を制御する遺伝子であるFCAは,RNA結合ドメインを含むタンパク質をコードする
R Macknight1, I Bancroft, T Page
1Department of Molecular Genetics, John Innes Centre, Norwich Research Park, Colney, United Kingdom.
Cell
|May 30, 1997
まとめ
FCA遺伝子は,RNAを調節することによって,アラビドプシスの花期を促進します. 過剰発現したFCAは花開を加速し,FCAのトランスクリプトレベルを変化させ,トランスクリプション後の制御におけるその役割を示している.
科学分野:
- 植物生物学 植物生物学
- 分子遺伝学 分子遺伝学
- 発達生物学 発達生物学とは
背景:
- 咲く時期は植物における重要な発達過程であり,遺伝的および環境的要因の影響を受けます.
- FCA遺伝子は,アラビドプシス・タリアナの開花への移行のレギュレータとして知られています.
- FCAは,RNA結合およびタンパク質相互作用ドメインを持つタンパク質をコードし,転写後の遺伝子調節における役割を示唆しています.
研究 の 目的:
- 花開の時間を調節するFCA遺伝子の機能を調査する.
- FCAが開花への移行を制御する分子メカニズムを理解する.
主な方法:
- FCA遺伝子のクローニングと,そのタンパク質製品の特徴づけ.
- FCAトランスクリプトのスプライシングと豊富さの分析.
- FCA遺伝子を35S構成プロモーターに融合させることによる遺伝子操作.
主要な成果:
- FCAは,RNA結合ドメインとWWドメインを持つタンパク質をコードし,転写後の調節におけるその役割をサポートします.
- FCAトランスクリプトの代替スプライシングが行われ,完全なタンパク質をコードする形式は1つのみである.
- 35Sプロモーターを使用したFCAの過剰発現は,アラビドプシスの早期開花につながった.
- FCAを過剰に発現する植物では,FCAトランスクリプトの変化した比率と豊富さが観察されました.
結論:
- FCAは,アラビドopsisの花開の時期の遺伝的コントロールの重要な要素です.
- FCAタンパク質は,花開を制御するポストトランスクリプションの規制カスケードで機能する可能性が高い.
- FCAの代替スプライシングとトランスクリプトの豊富さは,その規制役割の重要な側面です.
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