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Updated: Aug 7, 2026

14:57
Yeast As a Chassis for Developing Functional Assays to Study Human P53
Published on: August 4, 2019
p300/CBPコアクティベータによるp53の結合と調節
N L Lill1, S R Grossman, D Ginsberg
1The Dana-Farber Cancer Institute, Boston, Massachusetts 02115, USA.
Nature
|June 19, 1997
まとめ
ウイルス性オンコタンパク質は,p300/CBP結合に干渉することによって,p53の機能を破壊する. この相互作用はp53にとって極めて重要です.
科学分野:
- 分子生物学は分子生物学である.
- 細胞生物学 細胞生物学
- ウイルス学 ウイルス学 ウイルス学
背景:
- アデノウイルスE1AとSV40の大型T抗原オンコタンパク質は,p300/CBPの転写共同活性化剤と相互作用する.
- この相互作用は,細胞の変容メカニズムと関連しています.
- T抗原に共通する結合領域は,p53とp300/CBP機能の関連性を示唆している.
研究 の 目的:
- p300/CBPとp53.5の間の直接的な相互作用を調査する.
- この相互作用がp53を媒介する細胞プロセスに及ぼす機能的影響を決定する.
- p300/CBPがp53活動の媒介者として果たす役割を明らかにする.
主な方法:
- 共同免疫プレシピテーションは,タンパク質とタンパク質の相互作用を検出するための測定法です.
- 免疫光顕微鏡では,サブセルラー局所化を評価します.
- レポーター遺伝子の測定は,プロモーターの活性化を測定するために行われます.
- 細胞サイクル分析とアポトーシス分析は,機能的アウトカムを評価するために行われます.
主要な成果:
- p300/CBPは,ウイルスのオンコタンパク質がない場合,p53に直接結合する.
- p300とp53は核にコロカライズされ,安定したDNA結合複合体を形成する.
- アデノウイルスE1Aは,p21およびbaxプロモーターのp53-媒介の転写活性化を妨害する.
- E1Aは,p53-誘発のG1細胞サイクル停止とアポトーシスを抑制する.
結論:
- p300/CBPは,p53.5の転写アダプタとして作用する.
- p300/CBPは,G1細胞サイクルチェックポイント制御とアポトーシス誘導におけるp53の役割を調節する.
- ウイルスのオンコタンパク質によるp300/p53複合体の破壊は,細胞の変容に寄与する.
関連する概念動画
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These groups modify specific amino acids in a protein.
These groups modify specific amino acids in a protein.
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Proteins can undergo many types of post-translational modifications, often in response to changes in their environment. These modifications play an important role in the function and stability of these proteins. Covalently linked molecules include functional groups, such as methyl, acetyl, and phosphate groups, and also small proteins, such as ubiquitin. There are around 200 different types of covalent regulators that have been identified.
These groups modify specific amino acids in a protein.
These groups modify specific amino acids in a protein.
DNA Damage Can Stall the Cell Cycle
In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...

